A simple quantitative method for analyzing electrographic status epilepticus in rats

M. J. Lehmkuhle, K. E. Thomson, P. Scheerlinck, W. Pouliot, Bradley Greger, F. E. Dudek

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Electrographic status epilepticus (ESE) is a medical emergency consisting of repetitive seizures and may result in death or severe brain damage. Epilepsy can develop following ESE. The properties of ESE (e.g., duration and intensity) are variable, as are the effects of putative therapeutic treatments. Therefore a straightforward method to quantify different components of ESE would be beneficial for both researchers and clinicians. A frequency range close to the gamma band was selected for extraction of seizure-related activity from the EEG. This filtering strategy reduced motion artifacts and other noise sources in the electrophysiological recordings, thus increasing the signal-to-noise ratio of the EEG spike activity. EEG spiking was quantified using an energy operator and modeled by an eighth-order polynomial. In a benzodiazepine-resistant rat model of pilocarpine-induced ESE, the efficacy of various pharmaceutical agents at suppressing ESE was analyzed with this and other methods on data collected for ≤24 h after ESE induction. This approach allows for the objective, quantitative, and rapid assessment of the effects of both short- and long-lasting pharmacological manipulations on ESE and other forms of prolonged repetitive electrical activity.

Original languageEnglish (US)
Pages (from-to)1660-1670
Number of pages11
JournalJournal of Neurophysiology
Volume101
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Status Epilepticus
Electroencephalography
Seizures
Pilocarpine
Signal-To-Noise Ratio
Therapeutic Uses
Benzodiazepines
Artifacts
Noise
Epilepsy
Emergencies
Research Personnel
Pharmacology
Brain
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Lehmkuhle, M. J., Thomson, K. E., Scheerlinck, P., Pouliot, W., Greger, B., & Dudek, F. E. (2009). A simple quantitative method for analyzing electrographic status epilepticus in rats. Journal of Neurophysiology, 101(3), 1660-1670. https://doi.org/10.1152/jn.91062.2008

A simple quantitative method for analyzing electrographic status epilepticus in rats. / Lehmkuhle, M. J.; Thomson, K. E.; Scheerlinck, P.; Pouliot, W.; Greger, Bradley; Dudek, F. E.

In: Journal of Neurophysiology, Vol. 101, No. 3, 03.2009, p. 1660-1670.

Research output: Contribution to journalArticle

Lehmkuhle, MJ, Thomson, KE, Scheerlinck, P, Pouliot, W, Greger, B & Dudek, FE 2009, 'A simple quantitative method for analyzing electrographic status epilepticus in rats', Journal of Neurophysiology, vol. 101, no. 3, pp. 1660-1670. https://doi.org/10.1152/jn.91062.2008
Lehmkuhle, M. J. ; Thomson, K. E. ; Scheerlinck, P. ; Pouliot, W. ; Greger, Bradley ; Dudek, F. E. / A simple quantitative method for analyzing electrographic status epilepticus in rats. In: Journal of Neurophysiology. 2009 ; Vol. 101, No. 3. pp. 1660-1670.
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