A simple multiplex polymerase chain reaction to determine ABO blood types of rhesus macaques (Macaca mulatta)

A. Premasuthan, S. Kanthaswamy, J. Satkoski, D. G. Smith

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Rhesus macaques are the most common nonhuman primate model organism used in biomedical research. Their increasingly frequent use as subjects in studies involving transplantation requires that blood and other tissue antigens of donors and recipients be compatible. We report here an easy and rapid multiplex polymerase chain reaction (PCR) to determine the ABO blood group phenotypes of rhesus macaques that can be performed with only small amounts of DNA. We phenotyped 78 individuals and found this species to exhibit the A, B and AB phenotypes in frequencies that vary by geographic region. The probability of randomly pairing rhesus macaque donors and recipients that exhibit major ABO phenotype incompatibility is approximately 0.35 and 0.45 for Indian and Chinese rhesus macaques, respectively.

Original languageEnglish (US)
Pages (from-to)584-588
Number of pages5
JournalTissue antigens
Volume77
Issue number6
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Keywords

  • ABO
  • Blood type
  • Multiplex polymerase chain reaction
  • Rhesus macaque

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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