A series of IncQ-based reporter plasmids for use in a range of gram-negative genera

Laura E. O'Sullivan, Cheryl Nickerson, James W. Wilson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.

Original languageEnglish (US)
Pages (from-to)871-874
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume20
Issue number5
DOIs
Publication statusPublished - May 2010

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Keywords

  • Broad host range
  • IncP
  • IncQ plasmid
  • PQlacZ
  • pUZ8002

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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