TY - JOUR
T1 - A series of IncQ-based reporter plasmids for use in a range of gram-negative genera
AU - O'Sullivan, Laura E.
AU - Nickerson, Cheryl
AU - Wilson, James W.
PY - 2010/5
Y1 - 2010/5
N2 - Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.
AB - Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.
KW - Broad host range
KW - IncP
KW - IncQ plasmid
KW - PQlacZ
KW - pUZ8002
UR - http://www.scopus.com/inward/record.url?scp=77954974482&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954974482&partnerID=8YFLogxK
U2 - 10.4014/jmb.0912.12017
DO - 10.4014/jmb.0912.12017
M3 - Article
C2 - 20519909
AN - SCOPUS:77954974482
SN - 1017-7825
VL - 20
SP - 871
EP - 874
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 5
ER -