A sensitive electrochemical protein quantification method

Alexandra Schwarz, Olivier Bagel, Hubert H. Girault

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

This article presents a sensitive electrochemical method for the quantification of proteins. The assay used the biuret reaction, which is based on the complexation of proteins with copper ions to form a copper-protein complex. In the present approach, once copper ions were complexed with the protein, they were released by an acidic treatment and simultaneously separated by ultracentrifugation from the protein sample. This allowed differential pulse anodic stripping voltammetry (DPASV) of Cu2+ on carbon rotogravure printed electrodes (CARPEs). A nM detection limit for bovine serum albumin (BSA) was found with this method. The kinetics of this assay were tested and it resulted that the total assay can be carried out in 27 min, including incubation, washing steps and detection.

Original languageEnglish (US)
Pages (from-to)811-815
Number of pages5
JournalElectroanalysis
Volume12
Issue number11
DOIs
StatePublished - Dec 14 2000
Externally publishedYes

Keywords

  • Biuret reaction
  • Copper
  • Electrochemical detection
  • Protein assay

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Fingerprint Dive into the research topics of 'A sensitive electrochemical protein quantification method'. Together they form a unique fingerprint.

Cite this