A secreted high-affinity inhibitor of human TNF from Tanapox virus

Craig R. Brunetti, Mini Paulose-Murphy, Rajkumari Singh, Jing Qin, John W. Barrett, Aubry Tardivel, Pascal Schneider, Karim Essani, Douglas McFadden

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A class of secreted poxvirus tumor necrosis factor (TN)-binding proteins has been isolated from Tanapox-infected cell supernatants. The inhibitor bound to a TN-affinity column and was identified as the product of the 2L gene. Sequence analysis of 2L family members from other yatapoxviruses and swinepox virus yielded no sequence homology to any known cellular gene. The expressed Tanapox virus 2L protein bound to human TN with high affinity (Kd = 43 pM) and exhibits an unusually slow off-rate. However, 2L is unable to bind to a wide range of human TN family members. The 2L protein can inhibit human TN from binding to TN receptors I and II as well as block TN-induced cytolysis. Thus, Tanapox virus 2L represents an inhibitor of human TN and offers a unique strategy with which to modulate TN activity.

Original languageEnglish (US)
Pages (from-to)4831-4836
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number8
DOIs
StatePublished - Apr 15 2003
Externally publishedYes

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Yatapoxvirus
Tumor Necrosis Factor-alpha
Suipoxvirus
Poxviridae
Tumor Necrosis Factor Receptors
Sequence Homology
Genes
Sequence Analysis
Carrier Proteins
Proteins
human TNF protein

ASJC Scopus subject areas

  • General

Cite this

A secreted high-affinity inhibitor of human TNF from Tanapox virus. / Brunetti, Craig R.; Paulose-Murphy, Mini; Singh, Rajkumari; Qin, Jing; Barrett, John W.; Tardivel, Aubry; Schneider, Pascal; Essani, Karim; McFadden, Douglas.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 8, 15.04.2003, p. 4831-4836.

Research output: Contribution to journalArticle

Brunetti, CR, Paulose-Murphy, M, Singh, R, Qin, J, Barrett, JW, Tardivel, A, Schneider, P, Essani, K & McFadden, D 2003, 'A secreted high-affinity inhibitor of human TNF from Tanapox virus', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 8, pp. 4831-4836. https://doi.org/10.1073/pnas.0737244100
Brunetti, Craig R. ; Paulose-Murphy, Mini ; Singh, Rajkumari ; Qin, Jing ; Barrett, John W. ; Tardivel, Aubry ; Schneider, Pascal ; Essani, Karim ; McFadden, Douglas. / A secreted high-affinity inhibitor of human TNF from Tanapox virus. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 8. pp. 4831-4836.
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AU - Schneider, Pascal

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