A scalable high-throughput chemical synthesizer

Eric A. Livesay, Ying Horng Liu, Kevin J. Luebke, Joel Irick, Yuri Belosludtsev, Simon Rayner, Robert Balog, Stephen Albert Johnston

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.

Original languageEnglish (US)
Pages (from-to)1950-1960
Number of pages11
JournalGenome research
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2002

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ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Livesay, E. A., Liu, Y. H., Luebke, K. J., Irick, J., Belosludtsev, Y., Rayner, S., Balog, R., & Johnston, S. A. (2002). A scalable high-throughput chemical synthesizer. Genome research, 12(12), 1950-1960. https://doi.org/10.1101/gr.359002