A real-time protein detector utilizing the Vroman effect on SAM-functionalied surfaces

Seokheun Choi, Yongmo Yang, Junseok Chae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We report a new SPR (surface plasmon resonance) protein sensor using the Vroman effect for real-time, sensitive and selective detection of protein. The sensor relies on the competitive nature of protein adsorption to the surface, directly depending upon protein’s molecular weight. We, for the first time, utilize the Vroman effect for real-time protein microsensors using three different proteins including annexin (36 kDa), streptavidine (53 kDa), and isolectin (114 kDa) on three different surfaces which are a bare-gold surface and two others modified by OH-and COOH-terminated SAM (Self Assembly Monolayer). The real-time adsorption and displacement of the proteins are monitored by SPR demonstrating very high sensitivity and selectivity label-free protein sensor. The biosensor can distinguish at least 17 kDa in molecular weight with 100 % selectivity. The protein detector can be integrated with microfluidic systems to provide extremely sensitive and selective analytical capability.

Original languageEnglish (US)
Title of host publication2008 Solid-State Sensors, Actuators, and Microsystems Workshop
EditorsKimberly L. Turner, Leland Spangler
PublisherTransducer Research Foundation
Pages138-141
Number of pages4
ISBN (Electronic)0964002477, 9780964002470
DOIs
StatePublished - 2008
Event2008 Solid-State Sensors, Actuators, and Microsystems Workshop - Hilton Head Island, United States
Duration: Jun 1 2008Jun 5 2008

Publication series

NameTechnical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop

Conference

Conference2008 Solid-State Sensors, Actuators, and Microsystems Workshop
CountryUnited States
CityHilton Head Island
Period6/1/086/5/08

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Hardware and Architecture

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    Choi, S., Yang, Y., & Chae, J. (2008). A real-time protein detector utilizing the Vroman effect on SAM-functionalied surfaces. In K. L. Turner, & L. Spangler (Eds.), 2008 Solid-State Sensors, Actuators, and Microsystems Workshop (pp. 138-141). (Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop). Transducer Research Foundation. https://doi.org/10.31438/trf.hh2008.38