A rapid quantitative real-time PCR-based DNA quantification assay coupled with species-assignment capabilities for two hybridizing macaca species

A. Barr, A. Premasuthan, J. Satkoski, D. G. Smith, D. George, S. Kanthaswamy

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Regional populations of rhesus and long-tailed macaques exhibit fundamental differences in mitochondrial DNA, short tandem repeat and single nucleotide polymorphism variation between mainland and insular Southeast Asian populations. Some studies have revealed genetic admixture between these species due to natural hybridization and human-assisted intercrosses. A quantitative real-time PCR (qPCR) assay was developed to efficiently determine the species of origin of a macaque biological sample, and to quantify the species-specific template DNA. Prior knowledge of species identity and DNA concentrations are crucial for maintaining cost-effective methods and accurate DNA analysis. DNA from 109 regionally representative rhesus and long-tailed macaques was qPCR amplified to determine the species and template quantities. Of the 19 Vietnamese long-tailed macaques, 3 samples were discovered to be hybrids.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalFolia Primatologica
Volume82
Issue number2
DOIs
StatePublished - Oct 2011

Keywords

  • DNA quantification
  • Long-tailed macaques
  • Macaca fascicularis
  • Macaca mulatta
  • Rhesus macaques
  • Species identification
  • TaqMan assay

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

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