Abstract
A rapid dilution cuvette has been designed for measurement of the rates of microtubule disassembly and the factors which stabilize tubules against dilution-induced depolymerization. The device minimizes microtubule shearing by obviating the passage of microtubules through small orifices, and optical measurements of the turbidity changes during disassembly are optimized by use of a long-path-length configuration. Details on the design and application of the rapid dilution cuvette to probe microtubule disassembly are described here. In principle, the method may be applied to study any indefinite polymerization process which is characterized by critical concentration phenomena.
Original language | English (US) |
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Pages (from-to) | 311-314 |
Number of pages | 4 |
Journal | Analytical Biochemistry |
Volume | 104 |
Issue number | 2 |
DOIs | |
State | Published - May 15 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology