A rapid dilution cuvette for kinetic studies of microtubule disassembly

Timothy L. Karr, Daniel L. Purich

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A rapid dilution cuvette has been designed for measurement of the rates of microtubule disassembly and the factors which stabilize tubules against dilution-induced depolymerization. The device minimizes microtubule shearing by obviating the passage of microtubules through small orifices, and optical measurements of the turbidity changes during disassembly are optimized by use of a long-path-length configuration. Details on the design and application of the rapid dilution cuvette to probe microtubule disassembly are described here. In principle, the method may be applied to study any indefinite polymerization process which is characterized by critical concentration phenomena.

Original languageEnglish (US)
Pages (from-to)311-314
Number of pages4
JournalAnalytical Biochemistry
Volume104
Issue number2
DOIs
StatePublished - May 15 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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