A quantitative comparison of synaptic density following perfusion versus immersion fixation in the rat cerebral cortex

Marie H. Monfils; Doug F. Bray; Ira Driscoll; Jeffrey A. Kleim; Bryan Kolb

doi:10.1002/jemt.20214

A quantitative comparison of synaptic density following perfusion versus immersion fixation in the rat cerebral cortex

Marie H. Monfils, Doug F. Bray, Ira Driscoll, Jeffrey A. Kleim, Bryan Kolb

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The main objective of the present study was to perform an unbiased comparison of immersion vs. perfusion techniques to assess whether we could use the former to quantify synapses through electron microscopy (EM). Using the immersion technique is ideally suited for instances in which the specimen under study could not be perfused under the standard EM protocol. Our results suggest that, despite suboptimal qualitative results, fixation by immersion allows for adequate quantification of synapses.

Original language	English (US)
Pages (from-to)	300-304
Number of pages	5
Journal	Microscopy Research and Technique
Volume	67
Issue number	6
DOIs	https://doi.org/10.1002/jemt.20214
State	Published - Aug 15 2005
Externally published	Yes

Keywords

Electron microscopy
Golgi-Cox
Synaptic density

ASJC Scopus subject areas

Anatomy
Histology
Instrumentation
Medical Laboratory Technology

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10.1002/jemt.20214

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abstract = "The main objective of the present study was to perform an unbiased comparison of immersion vs. perfusion techniques to assess whether we could use the former to quantify synapses through electron microscopy (EM). Using the immersion technique is ideally suited for instances in which the specimen under study could not be perfused under the standard EM protocol. Our results suggest that, despite suboptimal qualitative results, fixation by immersion allows for adequate quantification of synapses.",

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AU - Driscoll, Ira

AU - Kleim, Jeffrey A.

AU - Kolb, Bryan

PY - 2005/8/15

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KW - Electron microscopy

KW - Golgi-Cox

KW - Synaptic density

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A quantitative comparison of synaptic density following perfusion versus immersion fixation in the rat cerebral cortex

Abstract

Keywords

ASJC Scopus subject areas

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