A quantitative comparison of synaptic density following perfusion versus immersion fixation in the rat cerebral cortex

Marie H. Monfils, Doug F. Bray, Ira Driscoll, Jeffrey A. Kleim, Bryan Kolb

Research output: Contribution to journalArticle

5 Scopus citations


The main objective of the present study was to perform an unbiased comparison of immersion vs. perfusion techniques to assess whether we could use the former to quantify synapses through electron microscopy (EM). Using the immersion technique is ideally suited for instances in which the specimen under study could not be perfused under the standard EM protocol. Our results suggest that, despite suboptimal qualitative results, fixation by immersion allows for adequate quantification of synapses.

Original languageEnglish (US)
Pages (from-to)300-304
Number of pages5
JournalMicroscopy Research and Technique
Issue number6
StatePublished - Aug 15 2005



  • Electron microscopy
  • Golgi-Cox
  • Synaptic density

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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