A poxvirus-encoded uracil DNA glycosylase is essential for virus viability

D. T. Stuart, C. Upton, M. A. Higman, E. G. Niles, Douglas McFadden

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Infection of cultured mammalian cells with the Leporipoxvirus Shope fibroma virus (SFV) causes the induction of a novel uracil DNA glycosylase activity in the cytoplasms of the infected cells. The induction of this activity, early in infection, correlates with the early expression of the SFV BamHI D6R open reading frame which possesses significant protein sequence similarity to eukaryotic and prokaryotic uracil DNA glycosylases. The SFV BamHI D6R open reading frame and the homologous HindIII D4R open reading frame from the Orthopoxvirus vaccinia virus were cloned under the regulation of a phage T7 promoter and expressed in Escherichia coli as insoluble high- molecular-weight aggregates. During electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, the E. coli-expressed proteins migrate with an apparent molecular mass of 25 kDa. The insoluble protein aggregate generated by expression in E. coli was solubilized in urea and, following a subsequent refolding step, displayed the ability to excise uracil residues from double- stranded plasmid DNA substrates, with the subsequent formation of apyrimidinic sites. The viral enzyme, like all other characterized uracil DNA glycosylases, is active in the presence of high concentrations of EDTA, is substrate inhibited by uracil, and does not display any endonuclease activity. Attempts to inactivate the HindIII D4R gene of vaccinia virus by targeted insertion of a dominant xanthine-guanine phosphoribosyltransferase selection marker or direct insertion of a frame-shifted oligonucleotide were uniformly unsuccessful demonstrating that, unlike the uracil DNA glycosylase described for herpesviruses, the poxvirus enzyme is essential for virus viability.

Original languageEnglish (US)
Pages (from-to)2503-2512
Number of pages10
JournalJournal of Virology
Volume67
Issue number5
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Poxviridae
Uracil-DNA Glycosidase
Microbial Viability
Rabbit fibroma virus
Rabbit Fibroma Virus
viability
Open Reading Frames
open reading frames
viruses
Vaccinia virus
uracil
Uracil
protein aggregates
Escherichia coli
Leporipoxvirus
Orthopoxvirus
molecular weight
Bacteriophage T7
Virus Activation
xanthine

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Stuart, D. T., Upton, C., Higman, M. A., Niles, E. G., & McFadden, D. (1993). A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. Journal of Virology, 67(5), 2503-2512.

A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. / Stuart, D. T.; Upton, C.; Higman, M. A.; Niles, E. G.; McFadden, Douglas.

In: Journal of Virology, Vol. 67, No. 5, 01.01.1993, p. 2503-2512.

Research output: Contribution to journalArticle

Stuart, DT, Upton, C, Higman, MA, Niles, EG & McFadden, D 1993, 'A poxvirus-encoded uracil DNA glycosylase is essential for virus viability', Journal of Virology, vol. 67, no. 5, pp. 2503-2512.
Stuart DT, Upton C, Higman MA, Niles EG, McFadden D. A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. Journal of Virology. 1993 Jan 1;67(5):2503-2512.
Stuart, D. T. ; Upton, C. ; Higman, M. A. ; Niles, E. G. ; McFadden, Douglas. / A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. In: Journal of Virology. 1993 ; Vol. 67, No. 5. pp. 2503-2512.
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