A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

Andrew N. Webber; Sean M. Hird; Leonard C. Packman; Tristan A. Dyer; John C. Gray

doi:10.1007/BF00020499

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

Andrew N. Webber, Sean M. Hird, Leonard C. Packman, Tristan A. Dyer, John C. Gray

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.

Original language	English (US)
Pages (from-to)	141-151
Number of pages	11
Journal	Plant Molecular Biology
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/BF00020499
State	Published - Feb 1989
Externally published	Yes

Keywords

chloroplast DNA
cytochrome b-559
photosystem II
psbL
wheat

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/BF00020499

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@article{e5210741012a489aa9616eaf9e0acf78,

title = "A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA",

abstract = "The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.",

keywords = "chloroplast DNA, cytochrome b-559, photosystem II, psbL, wheat",

author = "Webber, {Andrew N.} and Hird, {Sean M.} and Packman, {Leonard C.} and Dyer, {Tristan A.} and Gray, {John C.}",

year = "1989",

month = feb,

doi = "10.1007/BF00020499",

language = "English (US)",

volume = "12",

pages = "141--151",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Netherlands",

number = "2",

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TY - JOUR

T1 - A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

AU - Webber, Andrew N.

AU - Hird, Sean M.

AU - Packman, Leonard C.

AU - Dyer, Tristan A.

AU - Gray, John C.

PY - 1989/2

Y1 - 1989/2

N2 - The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.

AB - The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.

KW - chloroplast DNA

KW - cytochrome b-559

KW - photosystem II

KW - psbL

KW - wheat

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U2 - 10.1007/BF00020499

DO - 10.1007/BF00020499

M3 - Article

C2 - 24272793

AN - SCOPUS:0000530782

SN - 0167-4412

VL - 12

SP - 141

EP - 151

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 2

ER -

A photosystem II polypeptide is encoded by an open reading frame co-transcribed with genes for cytochrome b-559 in wheat chloroplast DNA

Abstract

Keywords

ASJC Scopus subject areas

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