A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine

Xiangmin Zhang, Wei Kong, Shamaila Ashraf, Roy Curtiss

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza virus polymerase subunits, and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters, we constructed a 23.6-kb eight-unit plasmid that contains all the required constituents to generate influenza virus in chicken cells. Our "one-plasmid" system generated higher titers of influenza virus in chicken cells than the "eight-plasmid" system, enabling a simpler approach for generating vaccine seeds. Our study identified plasmid size as a potential limiting factor affecting transfection efficiency and hence the influenza viral yield from chicken cells.

Original languageEnglish (US)
Pages (from-to)9296-9303
Number of pages8
JournalJournal of Virology
Volume83
Issue number18
DOIs
StatePublished - Sep 2009

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Influenza Vaccines
Orthomyxoviridae
influenza
Chickens
Cultured Cells
plasmids
Plasmids
vaccines
chickens
ribonucleoproteins
Ribonucleoproteins
transfection
Transfection
cells
Reverse Genetics
nucleoproteins
Nucleoproteins
RNA Viruses
Viral RNA
Genetic Promoter Regions

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine. / Zhang, Xiangmin; Kong, Wei; Ashraf, Shamaila; Curtiss, Roy.

In: Journal of Virology, Vol. 83, No. 18, 09.2009, p. 9296-9303.

Research output: Contribution to journalArticle

Zhang, Xiangmin ; Kong, Wei ; Ashraf, Shamaila ; Curtiss, Roy. / A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine. In: Journal of Virology. 2009 ; Vol. 83, No. 18. pp. 9296-9303.
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