A novel geminivirus identified in tomato and cleome plants sampled in Brazil

Rafaela S. Fontenele, Natalia S. Lamas, Cristiano Lacorte, Ana Luiza M. Lacerda, Arvind Varsani, Simone G. Ribeiro

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Viruses in the family Geminiviridae have single-stranded DNA genomes encapsulated in geminate icosahedral particles. High throughput sequencing (HTS) for metagenomic approaches are being extensively used for the identification of known and novel viruses. Using a HTS approach, we identified a novel geminivirus in a tomato (Solanum lycopersicum) sample and a Cleome sp. sample collected in the midwest region of Brazil. The genomes from the two samples share 99.96% identity and ∼61–63% to genomes in the genus Capulavirus. The novel virus has been tentatively named tomato associated geminivirus 1 (TaGV1). No visual symptoms were observed in the field tomato plant or in the inoculated Nicotiana benthamiana where the virus established a systemic infection. The replication associated protein of TaGV1 is most similar to that encoded by capulaviruses (sharing 62–70% identity), whereas the CP is most similar to that of tomato pseudo curly top virus (sharing ∼31% identity). In the TaGV1 positive Cleome sp. sample, begomovirus DNA A and B components were also detected sharing 96% and 90% sequence identity to cleome leaf crumple virus DNA A and B components, respectively. Using a HTS approach, we identified TaGV1 in tomato and Cleome sp. samples and this is the first report of a geminivirus that is non-begomovirus in Brazil.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalVirus research
Volume240
DOIs
StatePublished - Aug 15 2017

Keywords

  • Cleome sp
  • Geminivirdae
  • Solanum lycopersicum

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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    Fontenele, R. S., Lamas, N. S., Lacorte, C., Lacerda, A. L. M., Varsani, A., & Ribeiro, S. G. (2017). A novel geminivirus identified in tomato and cleome plants sampled in Brazil. Virus research, 240, 175-179. https://doi.org/10.1016/j.virusres.2017.08.007