A new highly selective fluorescent K+ sensor

Xianfeng Zhou; Fengyu Su; Yanqing Tian; Cody Youngbull; Roger H. Johnson; Deirdre Meldrum

doi:10.1021/ja207345s

A new highly selective fluorescent K⁺ sensor

Xianfeng Zhou, Fengyu Su, Yanqing Tian, Cody Youngbull, Roger H. Johnson, Deirdre Meldrum

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137 Scopus citations

Abstract

We describe the synthesis, properties, and application of a new fluorescent potassium chemosensor, KS2, for K⁺ sensing and imaging in live cells. By virtue of a strong electron-withdrawing group, 2-dicyanomethylene-3- cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF), with a triazacryptand ligand, the new sensor can respond to K⁺ up to 1.6 M. This is the first highly selective intracellular sensor suitable for sensing K⁺ over a broad and high concentration range. Confocal fluorescence microscopy has established the utility of KS2 for live-cell K⁺ detection. The application of KS2 combined with other sensors will be of great benefit for investigating cellular metabolism, detecting and diagnosing diseases including cancer, and monitoring responses to therapy.

Original language	English (US)
Pages (from-to)	18530-18533
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	46
DOIs	https://doi.org/10.1021/ja207345s
State	Published - Nov 23 2011

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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title = "A new highly selective fluorescent K+ sensor",

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AU - Zhou, Xianfeng

AU - Su, Fengyu

AU - Tian, Yanqing

AU - Youngbull, Cody

AU - Johnson, Roger H.

AU - Meldrum, Deirdre

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AB - We describe the synthesis, properties, and application of a new fluorescent potassium chemosensor, KS2, for K+ sensing and imaging in live cells. By virtue of a strong electron-withdrawing group, 2-dicyanomethylene-3- cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF), with a triazacryptand ligand, the new sensor can respond to K+ up to 1.6 M. This is the first highly selective intracellular sensor suitable for sensing K+ over a broad and high concentration range. Confocal fluorescence microscopy has established the utility of KS2 for live-cell K+ detection. The application of KS2 combined with other sensors will be of great benefit for investigating cellular metabolism, detecting and diagnosing diseases including cancer, and monitoring responses to therapy.

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A new highly selective fluorescent K⁺ sensor

Abstract

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