A new approach for measuring single-cell oxygen consumption rates

Timothy W. Molter; Mark R. Holl; Joseph M. Dragavon; Sarah C. McQuaide; Judith B. Anderson; A. Cody Young; Lloyd W. Burgess; Mary E. Lidstrom; Deirdre Meldrum

doi:10.1109/TASE.2007.909441

A new approach for measuring single-cell oxygen consumption rates

Timothy W. Molter, Mark R. Holl, Joseph M. Dragavon, Sarah C. McQuaide, Judith B. Anderson, A. Cody Young, Lloyd W. Burgess, Mary E. Lidstrom, Deirdre Meldrum

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.

Original language	English (US)
Pages (from-to)	32-40
Number of pages	9
Journal	IEEE Transactions on Automation Science and Engineering
Volume	5
Issue number	1
DOIs	https://doi.org/10.1109/TASE.2007.909441
State	Published - Jan 2008

Keywords

Biochemistry
Frequency-domain analysis
Image processing
Oxygen
Phosphorus compounds

ASJC Scopus subject areas

Control and Systems Engineering
Electrical and Electronic Engineering

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10.1109/TASE.2007.909441

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title = "A new approach for measuring single-cell oxygen consumption rates",

abstract = "A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.",

keywords = "Biochemistry, Frequency-domain analysis, Image processing, Oxygen, Phosphorus compounds",

author = "Molter, {Timothy W.} and Holl, {Mark R.} and Dragavon, {Joseph M.} and McQuaide, {Sarah C.} and Anderson, {Judith B.} and Young, {A. Cody} and Burgess, {Lloyd W.} and Lidstrom, {Mary E.} and Deirdre Meldrum",

note = "Funding Information: Manuscript received December 22, 2006; revised March 30, 2007. This paper was recommended for publication by Associate Editor Y. Hayashizaki and Editor M. Wang upon evaluation of the reviewers{\textquoteright} comments. The work of D. Meldrum and M. Lidstrom was supported in part by the National Institutes of Health NHGRI Centers of Excellence in Genomic Science under Grant 5 P50 HG002360.",

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doi = "10.1109/TASE.2007.909441",

language = "English (US)",

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AU - Holl, Mark R.

AU - Dragavon, Joseph M.

AU - McQuaide, Sarah C.

AU - Anderson, Judith B.

AU - Young, A. Cody

AU - Burgess, Lloyd W.

AU - Lidstrom, Mary E.

AU - Meldrum, Deirdre

N1 - Funding Information: Manuscript received December 22, 2006; revised March 30, 2007. This paper was recommended for publication by Associate Editor Y. Hayashizaki and Editor M. Wang upon evaluation of the reviewers’ comments. The work of D. Meldrum and M. Lidstrom was supported in part by the National Institutes of Health NHGRI Centers of Excellence in Genomic Science under Grant 5 P50 HG002360.

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N2 - A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.

AB - A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.

KW - Biochemistry

KW - Frequency-domain analysis

KW - Image processing

KW - Oxygen

KW - Phosphorus compounds

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A new approach for measuring single-cell oxygen consumption rates

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Keywords

ASJC Scopus subject areas

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