A new apparatus for serial crystallography at the Advanced Light Source

David Shapiro, R. Bruce Doak, John Spence, Dmitri Starodub, Uwe Weierstall, Henry Chapman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have designed and commissioned an apparatus for serial crystallography of hydrated proteins at the Advanced Light Source. Serial crystallography is a recently proposed method of imaging uncrystallized proteins at a third generation synchrotron source. This paper describes the design of the apparatus and results from the first experiment, which recorded x-ray diffraction patterns from 8 micron droplets containing photosystem 1 protein molecules.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6318
DOIs
StatePublished - 2006
EventDevelopments in X-Ray Tomography V - San Diego, CA, United States
Duration: Aug 15 2006Aug 17 2006

Other

OtherDevelopments in X-Ray Tomography V
CountryUnited States
CitySan Diego, CA
Period8/15/068/17/06

Fingerprint

Crystallography
Light sources
Proteins
Synchrotrons
Diffraction patterns
Imaging techniques
X rays
Molecules
Experiments

Keywords

  • Crystallography
  • Serial crystallography
  • X-ray diffraction
  • X-ray diffraction imaging
  • X-ray microscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Shapiro, D., Bruce Doak, R., Spence, J., Starodub, D., Weierstall, U., & Chapman, H. (2006). A new apparatus for serial crystallography at the Advanced Light Source. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6318). [63180L] https://doi.org/10.1117/12.680785

A new apparatus for serial crystallography at the Advanced Light Source. / Shapiro, David; Bruce Doak, R.; Spence, John; Starodub, Dmitri; Weierstall, Uwe; Chapman, Henry.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6318 2006. 63180L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shapiro, D, Bruce Doak, R, Spence, J, Starodub, D, Weierstall, U & Chapman, H 2006, A new apparatus for serial crystallography at the Advanced Light Source. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6318, 63180L, Developments in X-Ray Tomography V, San Diego, CA, United States, 8/15/06. https://doi.org/10.1117/12.680785
Shapiro D, Bruce Doak R, Spence J, Starodub D, Weierstall U, Chapman H. A new apparatus for serial crystallography at the Advanced Light Source. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6318. 2006. 63180L https://doi.org/10.1117/12.680785
Shapiro, David ; Bruce Doak, R. ; Spence, John ; Starodub, Dmitri ; Weierstall, Uwe ; Chapman, Henry. / A new apparatus for serial crystallography at the Advanced Light Source. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6318 2006.
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