A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β

V. S. Prakash Chaturvedula, Zhijie Gao, Sidney Hecht, Shannon H. Jones, David G I Kingston

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bioassay-directed fractionation of a n-hexane extract of Couepia polyandra using an assay to detect inhibitors of the lyase activity of DNA polymerase β resulted in the isolation of the new triterpene 3β,16β ,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and β-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase β lyase activity.

Original languageEnglish (US)
Pages (from-to)1463-1465
Number of pages3
JournalJournal of Natural Products
Volume66
Issue number11
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Couepia
Lyases
lyases
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
triterpenoids
Stigmasterol
Oleanolic Acid
oleanolic acid
Triterpenes
sitosterols
stigmasterol
Bioassay
Fractionation
Biological Assay
hexane
Assays
fractionation
bioassays
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Prakash Chaturvedula, V. S., Gao, Z., Hecht, S., Jones, S. H., & Kingston, D. G. I. (2003). A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β. Journal of Natural Products, 66(11), 1463-1465.

A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β. / Prakash Chaturvedula, V. S.; Gao, Zhijie; Hecht, Sidney; Jones, Shannon H.; Kingston, David G I.

In: Journal of Natural Products, Vol. 66, No. 11, 11.2003, p. 1463-1465.

Research output: Contribution to journalArticle

Prakash Chaturvedula, VS, Gao, Z, Hecht, S, Jones, SH & Kingston, DGI 2003, 'A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β', Journal of Natural Products, vol. 66, no. 11, pp. 1463-1465.
Prakash Chaturvedula, V. S. ; Gao, Zhijie ; Hecht, Sidney ; Jones, Shannon H. ; Kingston, David G I. / A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β. In: Journal of Natural Products. 2003 ; Vol. 66, No. 11. pp. 1463-1465.
@article{4ac31a1048974c04801eccf71ee0645d,
title = "A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β",
abstract = "Bioassay-directed fractionation of a n-hexane extract of Couepia polyandra using an assay to detect inhibitors of the lyase activity of DNA polymerase β resulted in the isolation of the new triterpene 3β,16β ,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and β-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase β lyase activity.",
author = "{Prakash Chaturvedula}, {V. S.} and Zhijie Gao and Sidney Hecht and Jones, {Shannon H.} and Kingston, {David G I}",
year = "2003",
month = "11",
language = "English (US)",
volume = "66",
pages = "1463--1465",
journal = "Journal of Natural Products",
issn = "0163-3864",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - A New Acylated Oleanane Triterpenoid from Couepia polyandra that Inhibits the Lyase Activity of DNA Polymerase β

AU - Prakash Chaturvedula, V. S.

AU - Gao, Zhijie

AU - Hecht, Sidney

AU - Jones, Shannon H.

AU - Kingston, David G I

PY - 2003/11

Y1 - 2003/11

N2 - Bioassay-directed fractionation of a n-hexane extract of Couepia polyandra using an assay to detect inhibitors of the lyase activity of DNA polymerase β resulted in the isolation of the new triterpene 3β,16β ,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and β-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase β lyase activity.

AB - Bioassay-directed fractionation of a n-hexane extract of Couepia polyandra using an assay to detect inhibitors of the lyase activity of DNA polymerase β resulted in the isolation of the new triterpene 3β,16β ,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and β-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase β lyase activity.

UR - http://www.scopus.com/inward/record.url?scp=0344981283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344981283&partnerID=8YFLogxK

M3 - Article

C2 - 14640519

AN - SCOPUS:0344981283

VL - 66

SP - 1463

EP - 1465

JO - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

IS - 11

ER -