A Mycobacterium leprae‐specific gene encoding an immunologically recognized 45 kDa protein

Tobias F.Rinke De Wit, Josephine E. Clark‐Curtiss, Feseha Abebe, Arend H.J. Kolk, Anneke A.M. Janson, Miranda van Agterveld, Jelle E.R. Thole

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12 Scopus citations

Abstract

By screening a Mycobacterium leprae lambda gt11 expression library with a serum from an Ethiopian lepromatous leprosy (LL) patient a clone was isolated (LL4) belonging to hybridization group III of a panel of previously isolated M. leprae clones. Members of this hybridization group encode a serologically recognized 45 kDa protein. The complete DNA sequences of the partially overlapping clones LL4 and L1 (hybridization group III) are presented and these revealed the presence of an open reading frame (ORF) predicting a protein with a molecular size of 42 448 Da. Southern hybridizations on total genomic DNA of M. Ieprae, Mycobacterium tuberculosis and eight atypical mycobacteria showed that the LL4 DNA fragment is specific for M. Ieprae DNA even under low‐stringency conditions. The M. Ieprae specificity of LL4 DNA was further confirmed by the polymerase chain reaction using four different sets of primers. Western blotting analyses showed that the M. Ieprae 45 kDa protein is frequently recognized by antibodies from leprosy patients and that this recognition is specific since no antibodies could be detected in sera of tuberculosis patients. T‐cell proliferation assays also demonstrated T‐cell recognition by leprosy patients and healthy contacts of the M. Ieprae 45 kDa protein. The specificity of the LL4 DNA region and the 45 kDa antigen that is encoded by hybridization group III could provide unique tools for the development of M. Ieprae‐specific immunological and DNA reagents.

Original languageEnglish (US)
Pages (from-to)829-838
Number of pages10
JournalMolecular Microbiology
Volume10
Issue number4
DOIs
StatePublished - Nov 1993

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    De Wit, T. F. R., Clark‐Curtiss, J. E., Abebe, F., Kolk, A. H. J., Janson, A. A. M., Agterveld, M. V., & Thole, J. E. R. (1993). A Mycobacterium leprae‐specific gene encoding an immunologically recognized 45 kDa protein. Molecular Microbiology, 10(4), 829-838. https://doi.org/10.1111/j.1365-2958.1993.tb00953.x