A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein

Tobias F. Rinke De Wit, Josephine E. Clark-Curtiss, Feseha Abebe, Arend H J Kolk, Anneke A M Janson, Miranda Van Agterveld, Jelle E R Thole

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

By screening a Mycobacterium leprae lambda gt11 expression library with a serum from an Ethiopian lepromatous leprosy (LL) patient a clone was isolated (LL4) belonging to hybridization group III of a panel of previously isolated M. leprae clones. Members of this hybridization group encode a serologically recognized 45 kDa protein. The complete DNA sequences of the partially overlapping clones LL4 and L1 (hybridization group III) are presented and these revealed the presence of an open reading frame (ORF) predicting a protein with a molecular size of 42 448Da. Southern hybridizations on total genomic DNA of M. leprae, Mycobacterium tuberculosis and eight atypical mycobacteria showed that the LL4 DNA fragment is specific for M. leprae DNA even under low-stringency conditions. The M. leprae specificity of LL4 DNA was further confirmed by the polymerase chain reaction using four different sets of primers. Western blotting analyses showed that the M. leprae 45 kDa protein is frequently recognized by antibodies from leprosy patients and that this recognition is specific since no antibodies could be detected in sera of tuberculosis patients. T-cell proliferation assays also demonstrated T-cell recognition by leprosy patients and healthy contacts of the M. leprae 45 KDa protein. The specificity of the LL4 DNA region and the 45 kDa antigen that is encoded by hybridization group III could provide unique tools for the development of M. leprae-specific immunological and DNA reagents.

Original languageEnglish (US)
Pages (from-to)829-838
Number of pages10
JournalMolecular Microbiology
Volume10
Issue number4
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

Mycobacterium leprae
Genes
DNA
Proteins
Clone Cells
Leprosy
T-Lymphocytes
Lepromatous Leprosy
Nontuberculous Mycobacteria
Antibodies
Serum
Mycobacterium tuberculosis
Open Reading Frames
Tuberculosis
Western Blotting
Cell Proliferation
Antigens
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Rinke De Wit, T. F., Clark-Curtiss, J. E., Abebe, F., Kolk, A. H. J., Janson, A. A. M., Van Agterveld, M., & Thole, J. E. R. (1993). A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein. Molecular Microbiology, 10(4), 829-838.

A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein. / Rinke De Wit, Tobias F.; Clark-Curtiss, Josephine E.; Abebe, Feseha; Kolk, Arend H J; Janson, Anneke A M; Van Agterveld, Miranda; Thole, Jelle E R.

In: Molecular Microbiology, Vol. 10, No. 4, 11.1993, p. 829-838.

Research output: Contribution to journalArticle

Rinke De Wit, TF, Clark-Curtiss, JE, Abebe, F, Kolk, AHJ, Janson, AAM, Van Agterveld, M & Thole, JER 1993, 'A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein', Molecular Microbiology, vol. 10, no. 4, pp. 829-838.
Rinke De Wit TF, Clark-Curtiss JE, Abebe F, Kolk AHJ, Janson AAM, Van Agterveld M et al. A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein. Molecular Microbiology. 1993 Nov;10(4):829-838.
Rinke De Wit, Tobias F. ; Clark-Curtiss, Josephine E. ; Abebe, Feseha ; Kolk, Arend H J ; Janson, Anneke A M ; Van Agterveld, Miranda ; Thole, Jelle E R. / A Mycobacterium leprae-specific gene encoding an immunologically recognized 45 kDa protein. In: Molecular Microbiology. 1993 ; Vol. 10, No. 4. pp. 829-838.
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