A multiplexed, electrochemical interface for gene-circuit-based sensors

Peivand Sadat Mousavi, Sarah J. Smith, Jenise B. Chen, Margot Karlikow, Aidan Tinafar, Clare Robinson, Wenhan Liu, Duo Ma, Alexander A. Green, Shana O. Kelley, Keith Pardee

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The field of synthetic biology has used the engineered assembly of synthetic gene networks to create a wide range of functions in biological systems. To date, gene-circuit-based sensors have primarily used optical proteins (for example, fluorescent, colorimetric) as reporter outputs, which has limited the potential to measure multiple distinct signals. Here we present an electrochemical interface that permits expanded multiplexed reporting for cell-free gene-circuit-based sensors. We have engineered a scalable system of reporter enzymes that cleave specific DNA sequences in solution, which results in an electrochemical signal when these newly liberated strands are captured at the surface of a nanostructured microelectrode. We describe the development of this interface and show its utility using a ligand-inducible gene circuit and toehold switch-based sensors by demonstrating the detection of multiple antibiotic resistance genes in parallel. This technology has the potential to expand the field of synthetic biology by providing an interface for materials, hardware and software.

Original languageEnglish (US)
JournalNature Chemistry
DOIs
StateAccepted/In press - Jan 1 2019
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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    Sadat Mousavi, P., Smith, S. J., Chen, J. B., Karlikow, M., Tinafar, A., Robinson, C., Liu, W., Ma, D., Green, A. A., Kelley, S. O., & Pardee, K. (Accepted/In press). A multiplexed, electrochemical interface for gene-circuit-based sensors. Nature Chemistry. https://doi.org/10.1038/s41557-019-0366-y