Abstract
The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-448 |
| Number of pages | 8 |
| Journal | Nucleic Acids Research |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
A Modified Uridine in the Anticodon of E. Coli. / Altman, Sidney.
In: Nucleic Acids Research, Vol. 3, No. 2, 1976, p. 441-448.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Modified Uridine in the Anticodon of E. Coli
AU - Altman, Sidney
PY - 1976
Y1 - 1976
N2 - The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.
AB - The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.
UR - http://www.scopus.com/inward/record.url?scp=0017255590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017255590&partnerID=8YFLogxK
U2 - 10.1093/nar/3.2.441
DO - 10.1093/nar/3.2.441
M3 - Article
C2 - 768924
AN - SCOPUS:0017255590
VL - 3
SP - 441
EP - 448
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 2
ER -