A Modified Uridine in the Anticodon of E. Coli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalNucleic Acids Research
Volume3
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Anticodon
Uridine
ribonuclease T(2)
Escherichia coli
Thin Layer Chromatography
Sulfur
Digestion

ASJC Scopus subject areas

  • Genetics

Cite this

A Modified Uridine in the Anticodon of E. Coli. / Altman, Sidney.

In: Nucleic Acids Research, Vol. 3, No. 2, 1976, p. 441-448.

Research output: Contribution to journalArticle

@article{ccd562cb31234930a80c96fed2b3bf6c,
title = "A Modified Uridine in the Anticodon of E. Coli",
abstract = "The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.",
author = "Sidney Altman",
year = "1976",
doi = "10.1093/nar/3.2.441",
language = "English (US)",
volume = "3",
pages = "441--448",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - A Modified Uridine in the Anticodon of E. Coli

AU - Altman, Sidney

PY - 1976

Y1 - 1976

N2 - The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.

AB - The anticodon of an ochre-suppressing derivative of E. coli tRNATyrL, previously identified as UUA, can contain a modified uridlne (U+) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anticodon contalnlng fragments. U+R is found in less than stolchiometric molar yields in preparations of tRNATyrL Su+Oc. The electrophoretlc mobility of U+P is the same as Up at pH 3.5 and PH 7.5. U+ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.

UR - http://www.scopus.com/inward/record.url?scp=0017255590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017255590&partnerID=8YFLogxK

U2 - 10.1093/nar/3.2.441

DO - 10.1093/nar/3.2.441

M3 - Article

VL - 3

SP - 441

EP - 448

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 2

ER -