Abstract
The ability of DNA topoisomerase I to promote insertions and deletions in vitro has been studied at nucleotide resolution for structurally diverse DNA substrates that uncouple the cleavage and ligation reactions of the enzyme. Topoisomerase I-mediated ligations afforded DNA duplexes having deletions and insertions with “branched” substrates and deletions up to 18 nucleotides in length with substrates containing nicks or gaps. In addition, a number of the acceptor substrates altered the preferred site of DNA cleavage, thereby increasing the diversity of accessible ligation products. Also demonstrated by the production of two “recombinant” duplexes from a single set of reactants was the potential for amplification of such alterations. These findings illustrate plausible mechanisms by which topoisomerase I-mediated illegitimate recombination may obtain at a molecular level.
Original language | English (US) |
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Pages (from-to) | 6120-6129 |
Number of pages | 10 |
Journal | Biochemistry |
Volume | 34 |
Issue number | 18 |
DOIs | |
State | Published - May 1 1995 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry