A Microfluidic Bioreporter System for Space Flight Monitoring

Bruce C. Towe; Rhett L. Martineau; Christopher G. Cooney; Matthew E. Piccini; Patrick Daydif; Valerie Stout

A Microfluidic Bioreporter System for Space Flight Monitoring

Bruce C. Towe, Rhett L. Martineau, Christopher G. Cooney, Matthew E. Piccini, Patrick Daydif, Valerie Stout

Life Sciences, School of (SOLS)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Metabolic changes of a continuously-fed bacterial microculture are monitored as a potential means of understanding the effects of the space environment on life. A hand-held plastic device supports and monitors the metabolism of a 100 μl microculture of E. coli. Both a semi-permeable microdialysis fiber and a gas-permeable microbore silicone hollow fiber membrane (HFM) are threaded through the microculture. Microliter quantity solutions flow through the lumen of the microdialysis fiber to extract metabolic wastes and to deliver nutrients. A carbon dioxide absorbance-based indicator flows through the lumen of the silicone HFM to supply oxygen, remove carbon dioxide, and monitor carbon dioxide production. Carbon dioxide production, which is tracked as an indicator of metabolic response, is monitored with an optical sensor that has a response time of 15 minutes and a sensitivity of ±0.6 mmHg. We evaluated the device using ultraviolet light as a test stressor for the instrumentation. We were able to detect changes in metabolic activity by varying dialysis feed rate and stressing the cells with UV.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Editors	R.S. Leder
Pages	2991-2994
Number of pages	4
Volume	4
State	Published - 2003
Event	A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico Duration: Sep 17 2003 → Sep 21 2003

Other

Other	A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Country/Territory	Mexico
City	Cancun
Period	9/17/03 → 9/21/03

Keywords

Bioreporter
Biosensor
Biosentinel
Continuous-culture
Microculture
Microdialysis
Microfluidic

ASJC Scopus subject areas

Bioengineering

Cite this

Towe, BC, Martineau, RL, Cooney, CG, Piccini, ME, Daydif, P & Stout, V 2003, A Microfluidic Bioreporter System for Space Flight Monitoring. in RS Leder (ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 4, pp. 2991-2994, A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Cancun, Mexico, 9/17/03.

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title = "A Microfluidic Bioreporter System for Space Flight Monitoring",

abstract = "Metabolic changes of a continuously-fed bacterial microculture are monitored as a potential means of understanding the effects of the space environment on life. A hand-held plastic device supports and monitors the metabolism of a 100 μl microculture of E. coli. Both a semi-permeable microdialysis fiber and a gas-permeable microbore silicone hollow fiber membrane (HFM) are threaded through the microculture. Microliter quantity solutions flow through the lumen of the microdialysis fiber to extract metabolic wastes and to deliver nutrients. A carbon dioxide absorbance-based indicator flows through the lumen of the silicone HFM to supply oxygen, remove carbon dioxide, and monitor carbon dioxide production. Carbon dioxide production, which is tracked as an indicator of metabolic response, is monitored with an optical sensor that has a response time of 15 minutes and a sensitivity of ±0.6 mmHg. We evaluated the device using ultraviolet light as a test stressor for the instrumentation. We were able to detect changes in metabolic activity by varying dialysis feed rate and stressing the cells with UV.",

keywords = "Bioreporter, Biosensor, Biosentinel, Continuous-culture, Microculture, Microdialysis, Microfluidic",

author = "Towe, {Bruce C.} and Martineau, {Rhett L.} and Cooney, {Christopher G.} and Piccini, {Matthew E.} and Patrick Daydif and Valerie Stout",

year = "2003",

language = "English (US)",

volume = "4",

pages = "2991--2994",

editor = "R.S. Leder",

booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

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AU - Towe, Bruce C.

AU - Martineau, Rhett L.

AU - Cooney, Christopher G.

AU - Piccini, Matthew E.

AU - Daydif, Patrick

AU - Stout, Valerie

PY - 2003

Y1 - 2003

N2 - Metabolic changes of a continuously-fed bacterial microculture are monitored as a potential means of understanding the effects of the space environment on life. A hand-held plastic device supports and monitors the metabolism of a 100 μl microculture of E. coli. Both a semi-permeable microdialysis fiber and a gas-permeable microbore silicone hollow fiber membrane (HFM) are threaded through the microculture. Microliter quantity solutions flow through the lumen of the microdialysis fiber to extract metabolic wastes and to deliver nutrients. A carbon dioxide absorbance-based indicator flows through the lumen of the silicone HFM to supply oxygen, remove carbon dioxide, and monitor carbon dioxide production. Carbon dioxide production, which is tracked as an indicator of metabolic response, is monitored with an optical sensor that has a response time of 15 minutes and a sensitivity of ±0.6 mmHg. We evaluated the device using ultraviolet light as a test stressor for the instrumentation. We were able to detect changes in metabolic activity by varying dialysis feed rate and stressing the cells with UV.

AB - Metabolic changes of a continuously-fed bacterial microculture are monitored as a potential means of understanding the effects of the space environment on life. A hand-held plastic device supports and monitors the metabolism of a 100 μl microculture of E. coli. Both a semi-permeable microdialysis fiber and a gas-permeable microbore silicone hollow fiber membrane (HFM) are threaded through the microculture. Microliter quantity solutions flow through the lumen of the microdialysis fiber to extract metabolic wastes and to deliver nutrients. A carbon dioxide absorbance-based indicator flows through the lumen of the silicone HFM to supply oxygen, remove carbon dioxide, and monitor carbon dioxide production. Carbon dioxide production, which is tracked as an indicator of metabolic response, is monitored with an optical sensor that has a response time of 15 minutes and a sensitivity of ±0.6 mmHg. We evaluated the device using ultraviolet light as a test stressor for the instrumentation. We were able to detect changes in metabolic activity by varying dialysis feed rate and stressing the cells with UV.

KW - Bioreporter

KW - Biosensor

KW - Biosentinel

KW - Continuous-culture

KW - Microculture

KW - Microdialysis

KW - Microfluidic

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M3 - Conference contribution

AN - SCOPUS:1542270804

VL - 4

SP - 2991

EP - 2994

BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

A2 - Leder, R.S.

T2 - A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Y2 - 17 September 2003 through 21 September 2003

ER -

A Microfluidic Bioreporter System for Space Flight Monitoring

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Keywords

ASJC Scopus subject areas

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