A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Jacob Knittel, Gayathri Srinivasan, Carlye Frisch, Nicholas Brookhouser, Sreedevi Raman, Albert Essuman, David A. Brafman

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

Original languageEnglish (US)
Article number101632
JournalSTAR Protocols
Volume3
Issue number3
DOIs
StatePublished - Sep 16 2022

Keywords

  • Cell differentiation
  • Neuroscience
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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