A Mg2+-responding RNA that controls the expression of a Mg 2+ transporter

E. A. Groisman, M. J. Cromie, Yixin Shi, T. Latifi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mg2+ is the most abundant divalent cation in biological systems. It is required for ATP-mediated enzymatic reactions and as a stabilizer of ribosomes and membranes. The enteric bacterium Salmonella enterica serovar Typhimurium harbors three Mg2+ transporters and a regulatory system-termed PhoP/PhoQ-whose activity is regulated by the extracytoplasmic levels of Mg2+. We have determined that expression of the PhoP-activated Mg2+ transporter MgtA is also controlled by its 5′-untranslated region (5′UTR). The 5′UTR of the mgtA gene can adopt different stem-loop structures depending on the Mg2+ levels, which determine whether transcription reads through into the mgtA-coding region or stops within the 5′UTR. This makes the mgtA 5′UTR the first example of a cation-responding riboswitch. The initiation of mgtA transcription responds to extracytoplasmic Mg2+, and its elongation into the coding region to cytoplasmic Mg2+, which provides a singular example where the same ligand is sensed in different cellular compartments to regulate disparate steps in gene transcription. The PhoP-activated Mg2+ transporter MgtB is also regulated by Mg2+ in a strain lacking the Mg2+ sensor PhoQ, suggesting the presence of additional Mg 2+-responding devices.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume71
DOIs
StatePublished - 2006

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5' Untranslated Regions
5' untranslated regions
transporters
Transcription
RNA
transcription (genetics)
cations
Genes
Riboswitch
Salmonella
Salmonella enterica
Divalent Cations
enzymatic reactions
Biological systems
Enterobacteriaceae
Ports and harbors
ribosomes
Ribosomes
intestinal microorganisms
Salmonella Typhimurium

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biochemistry
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A Mg2+-responding RNA that controls the expression of a Mg 2+ transporter. / Groisman, E. A.; Cromie, M. J.; Shi, Yixin; Latifi, T.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 71, 2006, p. 251-258.

Research output: Contribution to journalArticle

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