PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.
|Original language||English (US)|
|Number of pages||4|
|Journal||Applied and environmental microbiology|
|State||Published - 1994|
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology