31 Citations (Scopus)

Abstract

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

Original languageEnglish (US)
Pages (from-to)1014-1017
Number of pages4
JournalApplied and Environmental Microbiology
Volume60
Issue number3
StatePublished - 1994

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Enterovirus
sewage sludge
Sewage
sludge
Soil
Polymerase Chain Reaction
soil
Enterovirus C
Poliovirus
resin
methodology
resins
enterovirus
method

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

A method to detect enteroviruses in sewage sludge-amended soil using the PCR. / Straub, T. M.; Pepper, I. L.; Abbaszadegan, Morteza; Gerba, C. P.

In: Applied and Environmental Microbiology, Vol. 60, No. 3, 1994, p. 1014-1017.

Research output: Contribution to journalArticle

Straub, T. M. ; Pepper, I. L. ; Abbaszadegan, Morteza ; Gerba, C. P. / A method to detect enteroviruses in sewage sludge-amended soil using the PCR. In: Applied and Environmental Microbiology. 1994 ; Vol. 60, No. 3. pp. 1014-1017.
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