A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization

Amy E. Emerson, Emily M. Slaby, Jessica D. Weaver

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

As the field of organoid development matures, the need to transplant organoids to evaluate and characterize their functionality grows. Decades of research developing islet organoid transplantation for the treatment of type 1 diabetes can contribute substantially to accelerating diverse tissue organoid transplantation. Biomaterials-based organoid delivery methods offer the potential to maximize organoid survival and engraftment. In this protocol, we describe a vasculogenic degradable hydrogel vehicle and a method to deliver organoids to intraperitoneal tissue. Further, we describe a method to fluorescently label and image functional vasculature within the graft as a tool to investigate organoid engraftment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-272
Number of pages14
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2258
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Engraftment
  • Organoids
  • Transplantation
  • Vascularization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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