@inbook{d83b85c634e243bea9c83a6371980e44,
title = "A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization",
abstract = "As the field of organoid development matures, the need to transplant organoids to evaluate and characterize their functionality grows. Decades of research developing islet organoid transplantation for the treatment of type 1 diabetes can contribute substantially to accelerating diverse tissue organoid transplantation. Biomaterials-based organoid delivery methods offer the potential to maximize organoid survival and engraftment. In this protocol, we describe a vasculogenic degradable hydrogel vehicle and a method to deliver organoids to intraperitoneal tissue. Further, we describe a method to fluorescently label and image functional vasculature within the graft as a tool to investigate organoid engraftment.",
keywords = "Engraftment, Organoids, Transplantation, Vascularization",
author = "Emerson, {Amy E.} and Slaby, {Emily M.} and Weaver, {Jessica D.}",
note = "Publisher Copyright: {\textcopyright} 2021, The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1174-6_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "259--272",
booktitle = "Methods in Molecular Biology",
}