A method for analysis of the detergent-resistant cytoskeleton of cells within organs

David Capco, David M. Munoz, Carl J. Gassmann

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We describe a method for the preparation of the detergent-resistant cytoskeleton and nuclear matrix of cells within organs and tissues. Such cells were previously inaccessible to study because the three-dimensional organization of cells in organs prevented uniform distribution of the detergent throughout the multiple cell layers. We use the method presented here to compare the proteins present in the cytoskeleton, nuclear matrix and soluble fractions of cells from different histotypes. SDS-gel analysis demonstrates that soluble and nuclear matrix proteins differ greatly between histotypes while cytoskeletal proteins are relatively similar. Immunocytochemical analysis of tissue prepared using this procedure also demonstrates that the intracellular structure of cells within organs differs from that of in vitro cultured cells.

Original languageEnglish (US)
Pages (from-to)607-616
Number of pages10
JournalTissue and Cell
Volume19
Issue number5
DOIs
StatePublished - 1987

Keywords

  • Cytoskeleton
  • chromatin
  • fibroblast
  • nuclear matrix
  • tissue culture

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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