Abstract

A three-electrode electrochemical biosensor has been developed using printed circuit boards for detecting bacterial contamination. This low-cost, small-size device consists of thin-film gold electrodes and is fabricated using photo-lithography paired with electro-deposition. Pathogen presence is deduced by detection of change in electrical impedance caused by binding of the pathogen to bio-receptors coated on the sensor surface. The biosensor has a total area of ~3.2 cm2 and requires only 100 µL of test sample for detection. The sensor geometry has been optimized using techniques from Design of Experiments, and the device can be operated using a small ac excitation potential of magnitude 5 mV. The sensor is tested on the common food-borne pathogen Salmonella typhimurium and is able detect bacterial concentrations of the order of 500 CFU/mL within 6 min. In this paper, the design and fabrication of the biosensor is detailed along with the experiments that validate its performance.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalIEEE Sensors Journal
Volume11
Issue number1
DOIs
StatePublished - 2011

Fingerprint

pathogens
Pathogens
bioinstrumentation
Biosensors
sensors
Sensors
Electrochemical electrodes
salmonella
Costs
Salmonella
Acoustic impedance
electrodes
experiment design
printed circuits
circuit boards
electrical impedance
food
Electrodeposition
electrodeposition
Printed circuit boards

Keywords

  • Biomedical transducers
  • design automation
  • electrochemical processes
  • impedance measurement

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

A Low-Cost Electrochemical Biosensor for Rapid Bacterial Detection. / Nandakumar, Vivek; Bishop, Daniel; Alonas, Eric; LaBelle, Jeffrey; Joshi, Lokesh; Alford, Terry.

In: IEEE Sensors Journal, Vol. 11, No. 1, 2011, p. 210-216.

Research output: Contribution to journalArticle

Nandakumar, V, Bishop, D, Alonas, E, LaBelle, J, Joshi, L & Alford, T 2011, 'A Low-Cost Electrochemical Biosensor for Rapid Bacterial Detection', IEEE Sensors Journal, vol. 11, no. 1, pp. 210-216. https://doi.org/10.1109/JSEN.2010.2055847
Nandakumar, Vivek ; Bishop, Daniel ; Alonas, Eric ; LaBelle, Jeffrey ; Joshi, Lokesh ; Alford, Terry. / A Low-Cost Electrochemical Biosensor for Rapid Bacterial Detection. In: IEEE Sensors Journal. 2011 ; Vol. 11, No. 1. pp. 210-216.
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