Abstract
A three-electrode electrochemical biosensor has been developed using printed circuit boards for detecting bacterial contamination. This low-cost, small-size device consists of thin-film gold electrodes and is fabricated using photo-lithography paired with electro-deposition. Pathogen presence is deduced by detection of change in electrical impedance caused by binding of the pathogen to bio-receptors coated on the sensor surface. The biosensor has a total area of ~3.2 cm2 and requires only 100 µL of test sample for detection. The sensor geometry has been optimized using techniques from Design of Experiments, and the device can be operated using a small ac excitation potential of magnitude 5 mV. The sensor is tested on the common food-borne pathogen Salmonella typhimurium and is able detect bacterial concentrations of the order of 500 CFU/mL within 6 min. In this paper, the design and fabrication of the biosensor is detailed along with the experiments that validate its performance.
Original language | English (US) |
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Pages (from-to) | 210-216 |
Number of pages | 7 |
Journal | IEEE Sensors Journal |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2011 |
Keywords
- Biomedical transducers
- design automation
- electrochemical processes
- impedance measurement
ASJC Scopus subject areas
- Instrumentation
- Electrical and Electronic Engineering