A living cell array (LCA) for multiparameter cell metabolism studies

Sarah C. McQuaide, Mark R. Holl, Lloyd Burgess, Timothy Molter, Joseph Dragavon, A. Cody Young, Timothy Strovas, Judith Anderson, Alex Jen, Brandon Karlsgodt, Mary Lidstrom, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.

Original languageEnglish (US)
Title of host publicationProceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Pages971-976
Number of pages6
DOIs
StatePublished - Dec 22 2006
Event1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 - Pisa, Italy
Duration: Feb 20 2006Feb 22 2006

Publication series

NameProceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Volume2006

Other

Other1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
CountryItaly
CityPisa
Period2/20/062/22/06

Keywords

  • Microfabrication
  • Oxygen barrier
  • Oxygen consumption
  • Platinum porphyrin
  • Single-cell

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    McQuaide, S. C., Holl, M. R., Burgess, L., Molter, T., Dragavon, J., Young, A. C., Strovas, T., Anderson, J., Jen, A., Karlsgodt, B., Lidstrom, M., & Meldrum, D. (2006). A living cell array (LCA) for multiparameter cell metabolism studies. In Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 (pp. 971-976). [1639217] (Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006; Vol. 2006). https://doi.org/10.1109/BIOROB.2006.1639217