A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera

Apidae)

Juergen Gadau, Christine U. Gerloff, Nadia Krüger, Helen Chan, Paul Schmid-Hempel, Anja Wille, Robert Page

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We constructed a linkage map of Bombus terrestris (Hymenoptera, Apidae) phase unknown. The map contains 79 markers (six microsatellite and 73 RAPD markers) in 21 linkage groups and spans over 953.1 cM. The minimal recombinational size of the B. terrestris genome was estimated to be 1073 cM. Using flow cytometry, the physical size of the haploid genome of B. terrestris was calculated to be 274 Mb. This is the second linkage map for a social insect species. Bombus terrestris has on average five times less recombinational events per kb than the honey bee Apis mellifera. Male haploidy, chromosome size, and eusociality can now be excluded as reasons for the high recombination frequency of Apis mellifera. Finally, the sex determination locus of B. terrestris was placed on the map using bulked segregant analysis.

Original languageEnglish (US)
Pages (from-to)234-242
Number of pages9
JournalHeredity
Volume87
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Sex Determination Analysis
Bombus terrestris
Hymenoptera
Bees
sex determination analysis
Apidae
linkage (genetics)
Haploidy
Apis mellifera
haploidy
chromosome mapping
Genome Size
Honey
genome
Microsatellite Repeats
Genetic Recombination
social insects
Insects
Flow Cytometry
linkage groups

Keywords

  • Bombus terrestris
  • Complementary sex determination
  • Diploid males
  • Linkage map
  • Mapping phase unknown

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)

Cite this

Gadau, J., Gerloff, C. U., Krüger, N., Chan, H., Schmid-Hempel, P., Wille, A., & Page, R. (2001). A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera: Apidae). Heredity, 87(2), 234-242. https://doi.org/10.1046/j.1365-2540.2001.00919.x

A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera : Apidae). / Gadau, Juergen; Gerloff, Christine U.; Krüger, Nadia; Chan, Helen; Schmid-Hempel, Paul; Wille, Anja; Page, Robert.

In: Heredity, Vol. 87, No. 2, 2001, p. 234-242.

Research output: Contribution to journalArticle

Gadau, J, Gerloff, CU, Krüger, N, Chan, H, Schmid-Hempel, P, Wille, A & Page, R 2001, 'A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera: Apidae)', Heredity, vol. 87, no. 2, pp. 234-242. https://doi.org/10.1046/j.1365-2540.2001.00919.x
Gadau, Juergen ; Gerloff, Christine U. ; Krüger, Nadia ; Chan, Helen ; Schmid-Hempel, Paul ; Wille, Anja ; Page, Robert. / A linkage analysis of sex determination in Bombus terrestris (L.) (Hymenoptera : Apidae). In: Heredity. 2001 ; Vol. 87, No. 2. pp. 234-242.
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