A larval ‘princess pheromone’ identifies future ant queens based on their juvenile hormone content

Clint Penick, Juergen Liebig

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Numerous studies have identified cuticular compounds that distinguish adult queens from workers in social insect colonies, but how future queens are identified at the larval stage is poorly understood. Nevertheless, the ability of workers to discriminate queen and worker larvae is necessary for them to regulate caste determination and queen production. In the ant Harpegnathos saltator, workers bite larvae to inhibit queen development, and we used biting as an assay to test how workers identify queens at the larval stage. The transfer of cuticular compounds from queen to worker larvae through direct physical contact (rubbing) or using a hexane extract both elicited biting. Gas chromatography revealed significant differences in cuticular hydrocarbon profiles of queen and worker larvae that could be induced by treatment with a juvenile hormone (JH) analogue. Finally, treatment of male larvae with a JH analogue also elicited worker biting, which suggests a direct connection between JH levels and the production of a larval queen signal. These results demonstrate that workers identify larval caste using a chemical signal present on the cuticle, a ‘princess pheromone’, that reflects endocrine changes associated with queen development. Based on the connection between JH levels and the production of a larval queen signal, we developed a model for caste determination in H. saltator that incorporates endocrine, pheromonal and behavioural control of caste development.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalAnimal Behaviour
Volume128
DOIs
StatePublished - Jun 1 2017

Fingerprint

juvenile hormones
pheromone
queen insects
pheromones
hormone
ant
caste
larva
larvae
caste determination
juvenile hormone analogs
insect colonies
social insect
social insects
cuticle
hexane
hydrocarbons
gas chromatography
Formicidae
assay

Keywords

  • caste determination
  • chemical signaling
  • Harpegnathos
  • juvenile hormone
  • larval pheromone
  • social insect

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Cite this

A larval ‘princess pheromone’ identifies future ant queens based on their juvenile hormone content. / Penick, Clint; Liebig, Juergen.

In: Animal Behaviour, Vol. 128, 01.06.2017, p. 33-40.

Research output: Contribution to journalArticle

@article{7f926058737546f1a132c0e0ea774cd5,
title = "A larval ‘princess pheromone’ identifies future ant queens based on their juvenile hormone content",
abstract = "Numerous studies have identified cuticular compounds that distinguish adult queens from workers in social insect colonies, but how future queens are identified at the larval stage is poorly understood. Nevertheless, the ability of workers to discriminate queen and worker larvae is necessary for them to regulate caste determination and queen production. In the ant Harpegnathos saltator, workers bite larvae to inhibit queen development, and we used biting as an assay to test how workers identify queens at the larval stage. The transfer of cuticular compounds from queen to worker larvae through direct physical contact (rubbing) or using a hexane extract both elicited biting. Gas chromatography revealed significant differences in cuticular hydrocarbon profiles of queen and worker larvae that could be induced by treatment with a juvenile hormone (JH) analogue. Finally, treatment of male larvae with a JH analogue also elicited worker biting, which suggests a direct connection between JH levels and the production of a larval queen signal. These results demonstrate that workers identify larval caste using a chemical signal present on the cuticle, a ‘princess pheromone’, that reflects endocrine changes associated with queen development. Based on the connection between JH levels and the production of a larval queen signal, we developed a model for caste determination in H. saltator that incorporates endocrine, pheromonal and behavioural control of caste development.",
keywords = "caste determination, chemical signaling, Harpegnathos, juvenile hormone, larval pheromone, social insect",
author = "Clint Penick and Juergen Liebig",
year = "2017",
month = "6",
day = "1",
doi = "10.1016/j.anbehav.2017.03.029",
language = "English (US)",
volume = "128",
pages = "33--40",
journal = "Animal Behaviour",
issn = "0003-3472",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - A larval ‘princess pheromone’ identifies future ant queens based on their juvenile hormone content

AU - Penick, Clint

AU - Liebig, Juergen

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Numerous studies have identified cuticular compounds that distinguish adult queens from workers in social insect colonies, but how future queens are identified at the larval stage is poorly understood. Nevertheless, the ability of workers to discriminate queen and worker larvae is necessary for them to regulate caste determination and queen production. In the ant Harpegnathos saltator, workers bite larvae to inhibit queen development, and we used biting as an assay to test how workers identify queens at the larval stage. The transfer of cuticular compounds from queen to worker larvae through direct physical contact (rubbing) or using a hexane extract both elicited biting. Gas chromatography revealed significant differences in cuticular hydrocarbon profiles of queen and worker larvae that could be induced by treatment with a juvenile hormone (JH) analogue. Finally, treatment of male larvae with a JH analogue also elicited worker biting, which suggests a direct connection between JH levels and the production of a larval queen signal. These results demonstrate that workers identify larval caste using a chemical signal present on the cuticle, a ‘princess pheromone’, that reflects endocrine changes associated with queen development. Based on the connection between JH levels and the production of a larval queen signal, we developed a model for caste determination in H. saltator that incorporates endocrine, pheromonal and behavioural control of caste development.

AB - Numerous studies have identified cuticular compounds that distinguish adult queens from workers in social insect colonies, but how future queens are identified at the larval stage is poorly understood. Nevertheless, the ability of workers to discriminate queen and worker larvae is necessary for them to regulate caste determination and queen production. In the ant Harpegnathos saltator, workers bite larvae to inhibit queen development, and we used biting as an assay to test how workers identify queens at the larval stage. The transfer of cuticular compounds from queen to worker larvae through direct physical contact (rubbing) or using a hexane extract both elicited biting. Gas chromatography revealed significant differences in cuticular hydrocarbon profiles of queen and worker larvae that could be induced by treatment with a juvenile hormone (JH) analogue. Finally, treatment of male larvae with a JH analogue also elicited worker biting, which suggests a direct connection between JH levels and the production of a larval queen signal. These results demonstrate that workers identify larval caste using a chemical signal present on the cuticle, a ‘princess pheromone’, that reflects endocrine changes associated with queen development. Based on the connection between JH levels and the production of a larval queen signal, we developed a model for caste determination in H. saltator that incorporates endocrine, pheromonal and behavioural control of caste development.

KW - caste determination

KW - chemical signaling

KW - Harpegnathos

KW - juvenile hormone

KW - larval pheromone

KW - social insect

UR - http://www.scopus.com/inward/record.url?scp=85018439204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018439204&partnerID=8YFLogxK

U2 - 10.1016/j.anbehav.2017.03.029

DO - 10.1016/j.anbehav.2017.03.029

M3 - Article

VL - 128

SP - 33

EP - 40

JO - Animal Behaviour

JF - Animal Behaviour

SN - 0003-3472

ER -