A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)

Douglas Lake, Toru Sugano, Yoh ichi Matsumoto, Yasuhiko Masuho, Eskild A. Petersen, Paul Feorino, Evan M. Hersh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A stable hybridoma producing anti-HIV human monoclonal antibody (HMCA) was generated by fusing CD3-depleted human splenic lymphocytes from an HIV sero-positive donor with the mouse myeloma cell line P3x63AgU1. The resultant hybridoma has been secreting IgG1, lambda chain for over nine months at a rate of 2.5 ug/106cells/day. The HMCA shows specific reactivity in ELISA using HIV-infected cell lysates. Immunofluorescence tests have indicated that this HMCA binds specifically to the surface of H9 and C3 HIV/HTLVIIIb infected cells, HIV/N1T infected CEM cells and to MoT cells infected with an HIV clinical isolate. Western blotting revealed recognition of glycoproteins 120 and 160kDa of HIV by the HMCA. Although this HMCA demonstrated no neutralizing activity, the production of an anti-HIV HMCA specific for glycoprotein 120kDa indicates the possibility that a neutralizing HMCA can be developed as further fusions with lymph nodes and spleens from HIV positive donors are performed.

Original languageEnglish (US)
JournalLife Sciences
Volume45
Issue number16
DOIs
StatePublished - 1989
Externally publishedYes

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Hybridomas
Viruses
HIV-1
Glycoproteins
Monoclonal Antibodies
HIV
Lymphocytes
Fusion reactions
Immunoglobulin G
Cells
Fluorescent Antibody Technique
Spleen
Lymph Nodes
Western Blotting
Enzyme-Linked Immunosorbent Assay
Cell Line

ASJC Scopus subject areas

  • Pharmacology

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A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1). / Lake, Douglas; Sugano, Toru; Matsumoto, Yoh ichi; Masuho, Yasuhiko; Petersen, Eskild A.; Feorino, Paul; M. Hersh, Evan.

In: Life Sciences, Vol. 45, No. 16, 1989.

Research output: Contribution to journalArticle

Lake, Douglas ; Sugano, Toru ; Matsumoto, Yoh ichi ; Masuho, Yasuhiko ; Petersen, Eskild A. ; Feorino, Paul ; M. Hersh, Evan. / A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1). In: Life Sciences. 1989 ; Vol. 45, No. 16.
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AU - Petersen, Eskild A.

AU - Feorino, Paul

AU - M. Hersh, Evan

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