A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)

Douglas Lake; Toru Sugano; Yoh ichi Matsumoto; Yasuhiko Masuho; Eskild A. Petersen; Paul Feorino; Evan M. Hersh

doi:10.1016/0024-3205(89)90030-1

A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)

Douglas Lake, Toru Sugano, Yoh ichi Matsumoto, Yasuhiko Masuho, Eskild A. Petersen, Paul Feorino, Evan M. Hersh

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A stable hybridoma producing anti-HIV human monoclonal antibody (HMCA) was generated by fusing CD3-depleted human splenic lymphocytes from an HIV sero-positive donor with the mouse myeloma cell line P3x63AgU1. The resultant hybridoma has been secreting IgG1, lambda chain for over nine months at a rate of 2.5 ug/10⁶cells/day. The HMCA shows specific reactivity in ELISA using HIV-infected cell lysates. Immunofluorescence tests have indicated that this HMCA binds specifically to the surface of H9 and C3 HIV/HTLVIIIb infected cells, HIV/N1T infected CEM cells and to MoT cells infected with an HIV clinical isolate. Western blotting revealed recognition of glycoproteins 120 and 160kDa of HIV by the HMCA. Although this HMCA demonstrated no neutralizing activity, the production of an anti-HIV HMCA specific for glycoprotein 120kDa indicates the possibility that a neutralizing HMCA can be developed as further fusions with lymph nodes and spleens from HIV positive donors are performed.

Original language	English (US)
Pages (from-to)	iii-x
Journal	Life Sciences
Volume	45
Issue number	16
DOIs	https://doi.org/10.1016/0024-3205(89)90030-1
State	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(89)90030-1

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title = "A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)",

abstract = "A stable hybridoma producing anti-HIV human monoclonal antibody (HMCA) was generated by fusing CD3-depleted human splenic lymphocytes from an HIV sero-positive donor with the mouse myeloma cell line P3x63AgU1. The resultant hybridoma has been secreting IgG1, lambda chain for over nine months at a rate of 2.5 ug/106cells/day. The HMCA shows specific reactivity in ELISA using HIV-infected cell lysates. Immunofluorescence tests have indicated that this HMCA binds specifically to the surface of H9 and C3 HIV/HTLVIIIb infected cells, HIV/N1T infected CEM cells and to MoT cells infected with an HIV clinical isolate. Western blotting revealed recognition of glycoproteins 120 and 160kDa of HIV by the HMCA. Although this HMCA demonstrated no neutralizing activity, the production of an anti-HIV HMCA specific for glycoprotein 120kDa indicates the possibility that a neutralizing HMCA can be developed as further fusions with lymph nodes and spleens from HIV positive donors are performed.",

author = "Douglas Lake and Toru Sugano and Matsumoto, {Yoh ichi} and Yasuhiko Masuho and Petersen, {Eskild A.} and Paul Feorino and {M. Hersh}, Evan",

note = "Funding Information: This work was supported by funds from Teijin",

year = "1989",

doi = "10.1016/0024-3205(89)90030-1",

language = "English (US)",

volume = "45",

pages = "iii--x",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "16",

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T1 - A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)

AU - Lake, Douglas

AU - Sugano, Toru

AU - Matsumoto, Yoh ichi

AU - Masuho, Yasuhiko

AU - Petersen, Eskild A.

AU - Feorino, Paul

AU - M. Hersh, Evan

N1 - Funding Information: This work was supported by funds from Teijin

PY - 1989

Y1 - 1989

N2 - A stable hybridoma producing anti-HIV human monoclonal antibody (HMCA) was generated by fusing CD3-depleted human splenic lymphocytes from an HIV sero-positive donor with the mouse myeloma cell line P3x63AgU1. The resultant hybridoma has been secreting IgG1, lambda chain for over nine months at a rate of 2.5 ug/106cells/day. The HMCA shows specific reactivity in ELISA using HIV-infected cell lysates. Immunofluorescence tests have indicated that this HMCA binds specifically to the surface of H9 and C3 HIV/HTLVIIIb infected cells, HIV/N1T infected CEM cells and to MoT cells infected with an HIV clinical isolate. Western blotting revealed recognition of glycoproteins 120 and 160kDa of HIV by the HMCA. Although this HMCA demonstrated no neutralizing activity, the production of an anti-HIV HMCA specific for glycoprotein 120kDa indicates the possibility that a neutralizing HMCA can be developed as further fusions with lymph nodes and spleens from HIV positive donors are performed.

AB - A stable hybridoma producing anti-HIV human monoclonal antibody (HMCA) was generated by fusing CD3-depleted human splenic lymphocytes from an HIV sero-positive donor with the mouse myeloma cell line P3x63AgU1. The resultant hybridoma has been secreting IgG1, lambda chain for over nine months at a rate of 2.5 ug/106cells/day. The HMCA shows specific reactivity in ELISA using HIV-infected cell lysates. Immunofluorescence tests have indicated that this HMCA binds specifically to the surface of H9 and C3 HIV/HTLVIIIb infected cells, HIV/N1T infected CEM cells and to MoT cells infected with an HIV clinical isolate. Western blotting revealed recognition of glycoproteins 120 and 160kDa of HIV by the HMCA. Although this HMCA demonstrated no neutralizing activity, the production of an anti-HIV HMCA specific for glycoprotein 120kDa indicates the possibility that a neutralizing HMCA can be developed as further fusions with lymph nodes and spleens from HIV positive donors are performed.

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U2 - 10.1016/0024-3205(89)90030-1

DO - 10.1016/0024-3205(89)90030-1

M3 - Article

C2 - 2554084

AN - SCOPUS:0024367292

SN - 0024-3205

VL - 45

SP - iii-x

JO - Life Sciences

JF - Life Sciences

IS - 16

ER -

A hybridoma producing human monoclonal antibody specific for glycoprotein 120kDa of human immunodeficiency virus (HIV-1)

Abstract

ASJC Scopus subject areas

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