A high-throughput microfluidic light controlling platform for biofuel producing photosynthetic microalgae analysis

H. S. Kim, T. L. Weiss, T. P. Devarenne, A. Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

We have developed a high-throughput microalgae screening platform capable of analyzing biomass production at different light conditions with single colony resolution. Combinations of different light intensities and day-night cycles resulting in 64 light conditions for each of 64 culture compartments were implemented on a single microfluidic chip by blocking the light from a single light source with different black color dye concentrations and by switching between water and black color dye, respectively. Five microalgae colony-trapping structures in each culture compartment allowed single-colony resolution growth and biofuel-production analysis. Oil-producing microalgae, Botryococcus braunii, were successfully cultured and characterized using this screening system.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages295-297
Number of pages3
StatePublished - Dec 1 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Biofuel
  • High-throughput analysis
  • Microalgae culture microsystem
  • Microfluidic light controlling platform
  • Photosynthetic micro-algae

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'A high-throughput microfluidic light controlling platform for biofuel producing photosynthetic microalgae analysis'. Together they form a unique fingerprint.

  • Cite this

    Kim, H. S., Weiss, T. L., Devarenne, T. P., & Han, A. (2010). A high-throughput microfluidic light controlling platform for biofuel producing photosynthetic microalgae analysis. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 295-297). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).