Abstract
A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 175-178 |
| Number of pages | 4 |
| Journal | BBA - General Subjects |
| Volume | 717 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 16 1982 |
| Externally published | Yes |
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Keywords
- Concanvalin A
- High performance liquid chromatography
- Rapid analysis
- Size exclusion
- Subunit
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Medicine(all)
Cite this
A high performance liquid chromatography assay for the rapid analysis of the subunit content of concanavalin A. / Sitrin, Robert D.; Antell, Laura; Griswold, Don E.; Bender, Paul E.; Greig, Russell G.; Poste, George.
In: BBA - General Subjects, Vol. 717, No. 1, 16.07.1982, p. 175-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high performance liquid chromatography assay for the rapid analysis of the subunit content of concanavalin A
AU - Sitrin, Robert D.
AU - Antell, Laura
AU - Griswold, Don E.
AU - Bender, Paul E.
AU - Greig, Russell G.
AU - Poste, George
PY - 1982/7/16
Y1 - 1982/7/16
N2 - A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.
AB - A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.
KW - Concanvalin A
KW - High performance liquid chromatography
KW - Rapid analysis
KW - Size exclusion
KW - Subunit
UR - http://www.scopus.com/inward/record.url?scp=0020485111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020485111&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(82)90395-6
DO - 10.1016/0304-4165(82)90395-6
M3 - Article
C2 - 7104387
AN - SCOPUS:0020485111
VL - 717
SP - 175
EP - 178
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 1
ER -