A high performance liquid chromatography assay for the rapid analysis of the subunit content of concanavalin A

Robert D. Sitrin; Laura Antell; Don E. Griswold; Paul E. Bender; Russell G. Greig; George Poste

doi:10.1016/0304-4165(82)90395-6

A high performance liquid chromatography assay for the rapid analysis of the subunit content of concanavalin A

Robert D. Sitrin, Laura Antell, Don E. Griswold, Paul E. Bender, Russell G. Greig, George Poste

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.

Original language	English (US)
Pages (from-to)	175-178
Number of pages	4
Journal	BBA - General Subjects
Volume	717
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(82)90395-6
State	Published - Jul 16 1982
Externally published	Yes

Keywords

Concanvalin A
High performance liquid chromatography
Rapid analysis
Size exclusion
Subunit

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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abstract = "A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.",

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AU - Sitrin, Robert D.

AU - Antell, Laura

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AU - Bender, Paul E.

AU - Greig, Russell G.

AU - Poste, George

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AB - A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.

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