A high performance liquid chromatography assay for the rapid analysis of the subunit content of concanavalin A

Robert D. Sitrin, Laura Antell, Don E. Griswold, Paul E. Bender, Russell G. Greig, George Poste

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A high performance liquid chromatography system is described which provides a rapid and convenient assay for the relative amounts of intact (26 000 dalton) and fragmented (14 000 and 12 000 dalton) subunits present in preparations of concanavalin A. Analyses were performed on an HPLC size exlusions column using either 8 M urea or 6 M guanidine hydrochloride as denaturing eluents. The efficiency and resolving power of this technique were confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This HPLC assay facilitated the monitoring of the purification of concanavalin A to prepare a homogeneous preparation necessary for its biological evaluation.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalBBA - General Subjects
Volume717
Issue number1
DOIs
StatePublished - Jul 16 1982
Externally publishedYes

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Keywords

  • Concanvalin A
  • High performance liquid chromatography
  • Rapid analysis
  • Size exclusion
  • Subunit

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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