A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence

R. N. DuBois, M. W. McLane, K. Ryder, L. F. Lau, D. Nathans

    Research output: Contribution to journalArticlepeer-review

    205 Scopus citations

    Abstract

    Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3H) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.

    Original languageEnglish (US)
    Pages (from-to)19185-19191
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume265
    Issue number31
    StatePublished - 1990

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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