A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence

Raymond N. DuBois, Michael W. McLane, Kevin Ryder, Lester F. Lau, Daniel Nathans

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3R) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.

Original languageEnglish (US)
Pages (from-to)19185-19191
Number of pages7
JournalJournal of Biological Chemistry
Volume265
Issue number31
StatePublished - Nov 5 1990
Externally publishedYes

Fingerprint

Nucleic Acid Repetitive Sequences
Nuclear Proteins
Histidine
Cysteine
Intercellular Signaling Peptides and Proteins
Complementary DNA
Zinc
Genes
Cells
Thymus
Cell Line
3T3 Cells
Macrophages
Cloning
Zinc Fingers
Transcription
Regulator Genes
Heavy Metals
Proline
Liver

ASJC Scopus subject areas

  • Biochemistry

Cite this

DuBois, R. N., McLane, M. W., Ryder, K., Lau, L. F., & Nathans, D. (1990). A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. Journal of Biological Chemistry, 265(31), 19185-19191.

A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. / DuBois, Raymond N.; McLane, Michael W.; Ryder, Kevin; Lau, Lester F.; Nathans, Daniel.

In: Journal of Biological Chemistry, Vol. 265, No. 31, 05.11.1990, p. 19185-19191.

Research output: Contribution to journalArticle

DuBois, RN, McLane, MW, Ryder, K, Lau, LF & Nathans, D 1990, 'A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence', Journal of Biological Chemistry, vol. 265, no. 31, pp. 19185-19191.
DuBois RN, McLane MW, Ryder K, Lau LF, Nathans D. A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. Journal of Biological Chemistry. 1990 Nov 5;265(31):19185-19191.
DuBois, Raymond N. ; McLane, Michael W. ; Ryder, Kevin ; Lau, Lester F. ; Nathans, Daniel. / A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 31. pp. 19185-19191.
@article{abc09ea2dd464a1097ce1241fe8b1de1,
title = "A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence",
abstract = "Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3R) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.",
author = "DuBois, {Raymond N.} and McLane, {Michael W.} and Kevin Ryder and Lau, {Lester F.} and Daniel Nathans",
year = "1990",
month = "11",
day = "5",
language = "English (US)",
volume = "265",
pages = "19185--19191",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "31",

}

TY - JOUR

T1 - A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence

AU - DuBois, Raymond N.

AU - McLane, Michael W.

AU - Ryder, Kevin

AU - Lau, Lester F.

AU - Nathans, Daniel

PY - 1990/11/5

Y1 - 1990/11/5

N2 - Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3R) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.

AB - Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3R) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.

UR - http://www.scopus.com/inward/record.url?scp=0025001736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025001736&partnerID=8YFLogxK

M3 - Article

C2 - 1699942

AN - SCOPUS:0025001736

VL - 265

SP - 19185

EP - 19191

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 31

ER -