A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence

R. N. DuBois, M. W. McLane, K. Ryder, L. F. Lau, D. Nathans

Research output: Contribution to journalArticle

201 Scopus citations

Abstract

Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3H) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by γ-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.

Original languageEnglish (US)
Pages (from-to)19185-19191
Number of pages7
JournalJournal of Biological Chemistry
Volume265
Issue number31
StatePublished - Nov 22 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence'. Together they form a unique fingerprint.

  • Cite this

    DuBois, R. N., McLane, M. W., Ryder, K., Lau, L. F., & Nathans, D. (1990). A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. Journal of Biological Chemistry, 265(31), 19185-19191.