A genome-wide view of Caenorhabditis elegans base-substitution mutation processes

Dee R. Denver, Peter C. Dolan, Larry J. Wilhelm, Way Sung, J. Ignacio Lucas-Lledó, Dana K. Howe, Samantha C. Lewis, Kazu Okamoto, W. Kelley Thomas, Michael Lynch, Charles F. Baer

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Knowledge of mutation processes is central to understanding virtually all evolutionary phenomena and the underlying nature of genetic disorders and cancers. However, the limitations of standard molecular mutation detection methods have historically precluded a genome-wide understanding of mutation rates and spectra in the nuclear genomes of multicellular organisms. We applied two high-throughput DNA sequencing technologies to identify and characterize hundreds of spontaneously arising base-substitution mutations in 10 Caenorhabditis elegans mutation-accumulation (MA)-line nuclear genomes. C. elegans mutation rate estimates were similar to previous calculations based on smaller numbers of mutations. Mutations were distributed uniformly within and among chromosomes and were not associated with recombination rate variation in the MA lines, suggesting that intragenomic variation in genetic hitchhiking and/or background selection are primarily responsible for the chromosomal distribution patterns of polymorphic nucleotides in C. elegans natural populations. A strong mutational bias from G/C to A/T nucleotides was detected in the MA lines, implicating oxidative DNA damage as a major endogenous mutagenic force in C. elegans. The observed mutational bias also suggests that the C. elegans nuclear genome cannot be at equilibrium because of mutation alone. Transversions dominate the spectrum of spontaneous mutations observed here, whereas transitions dominate patterns of allegedly neutral polymorphism in natural populations of C. elegans and many other animal species; this observation challenges the assumption that natural patterns of molecular variation in noncoding regions of the nuclear genome accurately reflect underlying mutation processes.

Original languageEnglish (US)
Pages (from-to)16310-16314
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number38
DOIs
StatePublished - Sep 22 2009
Externally publishedYes

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Caenorhabditis elegans
Genome
Mutation
Mutation Rate
Nucleotides
High-Throughput Nucleotide Sequencing
Inborn Genetic Diseases
Genetic Recombination
Population
DNA Damage
Chromosomes
Technology

Keywords

  • High-throughput DNA sequencing
  • Mutation accumulation

ASJC Scopus subject areas

  • General

Cite this

Denver, D. R., Dolan, P. C., Wilhelm, L. J., Sung, W., Lucas-Lledó, J. I., Howe, D. K., ... Baer, C. F. (2009). A genome-wide view of Caenorhabditis elegans base-substitution mutation processes. Proceedings of the National Academy of Sciences of the United States of America, 106(38), 16310-16314. https://doi.org/10.1073/pnas.0904895106

A genome-wide view of Caenorhabditis elegans base-substitution mutation processes. / Denver, Dee R.; Dolan, Peter C.; Wilhelm, Larry J.; Sung, Way; Lucas-Lledó, J. Ignacio; Howe, Dana K.; Lewis, Samantha C.; Okamoto, Kazu; Thomas, W. Kelley; Lynch, Michael; Baer, Charles F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 38, 22.09.2009, p. 16310-16314.

Research output: Contribution to journalArticle

Denver, DR, Dolan, PC, Wilhelm, LJ, Sung, W, Lucas-Lledó, JI, Howe, DK, Lewis, SC, Okamoto, K, Thomas, WK, Lynch, M & Baer, CF 2009, 'A genome-wide view of Caenorhabditis elegans base-substitution mutation processes', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 38, pp. 16310-16314. https://doi.org/10.1073/pnas.0904895106
Denver, Dee R. ; Dolan, Peter C. ; Wilhelm, Larry J. ; Sung, Way ; Lucas-Lledó, J. Ignacio ; Howe, Dana K. ; Lewis, Samantha C. ; Okamoto, Kazu ; Thomas, W. Kelley ; Lynch, Michael ; Baer, Charles F. / A genome-wide view of Caenorhabditis elegans base-substitution mutation processes. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 38. pp. 16310-16314.
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