A genetic approach for analyzing the pathway of lamb assembly into the outer membrane of escherichia coli

Rajeev Misra, Amarette Peterson, Thomas Ferenci, Thomas J. Silhavy

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Results presented in this study demonstrate that a mutation which inserts an additional tyrosine between the 2 tyrosines at residues 118 and 119 of mature LamB protein results in a temperature-dependent assembly defect. This defect leads to the accumulation of an intermediate at the restrictive temperature that is most likely an assembly-defective monomer. These monomers are rapidly degraded in the wild type (htrA+) strain, and the biphasic kinetics of this degradation indicate that the mutation affects the assembly process and not the final product, i.e. stable trimers. In addition, our data show that the temperature-dependent assembly defect in the mutant strain is reversible, and therefore the accumulated monomers represent a true assembly intermediate. Fractionation studies show that the monomers, which can be accumulated in htrA (degP) mutants at the restrictive temperature, are associated with the outer membrane, indicating that trimerization of LamB is not a prerequisite for localization.

Original languageEnglish (US)
Pages (from-to)13592-13597
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number21
StatePublished - 1991
Externally publishedYes

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Escherichia coli
Membranes
Monomers
Temperature
Defects
Mutation
Tyrosine
Fractionation
Degradation
Kinetics
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

A genetic approach for analyzing the pathway of lamb assembly into the outer membrane of escherichia coli. / Misra, Rajeev; Peterson, Amarette; Ferenci, Thomas; Silhavy, Thomas J.

In: Journal of Biological Chemistry, Vol. 266, No. 21, 1991, p. 13592-13597.

Research output: Contribution to journalArticle

Misra, Rajeev ; Peterson, Amarette ; Ferenci, Thomas ; Silhavy, Thomas J. / A genetic approach for analyzing the pathway of lamb assembly into the outer membrane of escherichia coli. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 21. pp. 13592-13597.
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