A G43 to U43 mutation in E. coli tRNATyrsu3+ which affects processing by RNase P

Paul J. Furdon; Cecilia Guerrier-takada; Sidney Altman

doi:10.1093/nar/11.5.1491

A G43 to U43 mutation in E. coli tRNA^Tyrsu₃⁺ which affects processing by RNase P

Paul J. Furdon, Cecilia Guerrier-takada, Sidney Altman

Research output: Contribution to journal › Article › peer-review

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Abstract

A novel mutation in the anticodon stem of E. coli tRNA₁^Tyrsu³⁺ (G43 to U43) has been characterized. The gene c⊕ding for the mutant tRNA, carried by phage Ø80DHA61.3 a derivative of Ø80psu₃₊su₀ , produces only 20% of mature suppressor tRNA as compared with Ø80psu₃. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRN+A coded for by Ø80DHA61.3 is processed by RNase P more slowly than the su^Ø₃. pre+cursor and does not form as stable an enzyme-substrate complex as does su₃, precursor. Ø80DHA61.3 also +contains a large d-eletion which begins in the spa-cer region between the su, gene and the su₃⁺. gene, extends through the su₀. gene and includes most of the repeated region following the tRNA genes.

Original language	English (US)
Pages (from-to)	1491-1505
Number of pages	15
Journal	Nucleic acids research
Volume	11
Issue number	5
DOIs	https://doi.org/10.1093/nar/11.5.1491
State	Published - Mar 11 1983
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "A G43 to U43 mutation in E. coli tRNATyrsu3+ which affects processing by RNase P",

abstract = "A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+ (G43 to U43) has been characterized. The gene c⊕ding for the mutant tRNA, carried by phage {\O}80DHA61.3 a derivative of {\O}80psu3+su0 , produces only 20% of mature suppressor tRNA as compared with {\O}80psu3. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRN+A coded for by {\O}80DHA61.3 is processed by RNase P more slowly than the su{\O}3. pre+cursor and does not form as stable an enzyme-substrate complex as does su3, precursor. {\O}80DHA61.3 also +contains a large d-eletion which begins in the spa-cer region between the su, gene and the su3+. gene, extends through the su0. gene and includes most of the repeated region following the tRNA genes.",

author = "Furdon, {Paul J.} and Cecilia Guerrier-takada and Sidney Altman",

note = "Funding Information: Acknowledgements: We wish to thank Dr. G. McCorkle for his suggestions concerning cloning and sequencing techniques. P.F. was supported by a USPHS pre-doctoral training grant. S.A. is the recipient of USPHS grant GM19422 and NSF grant PCM 79-04054.",

year = "1983",

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doi = "10.1093/nar/11.5.1491",

language = "English (US)",

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AU - Furdon, Paul J.

AU - Guerrier-takada, Cecilia

AU - Altman, Sidney

N1 - Funding Information: Acknowledgements: We wish to thank Dr. G. McCorkle for his suggestions concerning cloning and sequencing techniques. P.F. was supported by a USPHS pre-doctoral training grant. S.A. is the recipient of USPHS grant GM19422 and NSF grant PCM 79-04054.

PY - 1983/3/11

Y1 - 1983/3/11

N2 - A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+ (G43 to U43) has been characterized. The gene c⊕ding for the mutant tRNA, carried by phage Ø80DHA61.3 a derivative of Ø80psu3+su0 , produces only 20% of mature suppressor tRNA as compared with Ø80psu3. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRN+A coded for by Ø80DHA61.3 is processed by RNase P more slowly than the suØ3. pre+cursor and does not form as stable an enzyme-substrate complex as does su3, precursor. Ø80DHA61.3 also +contains a large d-eletion which begins in the spa-cer region between the su, gene and the su3+. gene, extends through the su0. gene and includes most of the repeated region following the tRNA genes.

AB - A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+ (G43 to U43) has been characterized. The gene c⊕ding for the mutant tRNA, carried by phage Ø80DHA61.3 a derivative of Ø80psu3+su0 , produces only 20% of mature suppressor tRNA as compared with Ø80psu3. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRN+A coded for by Ø80DHA61.3 is processed by RNase P more slowly than the suØ3. pre+cursor and does not form as stable an enzyme-substrate complex as does su3, precursor. Ø80DHA61.3 also +contains a large d-eletion which begins in the spa-cer region between the su, gene and the su3+. gene, extends through the su0. gene and includes most of the repeated region following the tRNA genes.

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A G43 to U43 mutation in E. coli tRNA^Tyrsu₃⁺ which affects processing by RNase P

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