A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Abhinav P. Acharya; Parsa M. Nafisi; Austin Gardner; Joanna L. MacKay; Kousik Kundu; Sanjay Kumar; Niren Murthy

doi:10.1039/c3cc44783a

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Abhinav P. Acharya, Parsa M. Nafisi, Austin Gardner, Joanna L. MacKay, Kousik Kundu, Sanjay Kumar, Niren Murthy

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Original language	English (US)
Pages (from-to)	10379-10381
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	88
DOIs	https://doi.org/10.1039/c3cc44783a
State	Published - Oct 10 2013
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.",

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AU - Acharya, Abhinav P.

AU - Nafisi, Parsa M.

AU - Gardner, Austin

AU - MacKay, Joanna L.

AU - Kundu, Kousik

AU - Kumar, Sanjay

AU - Murthy, Niren

PY - 2013/10/10

Y1 - 2013/10/10

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AB - The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

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JF - Chemical Communications

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A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Abstract

ASJC Scopus subject areas

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