A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Abhinav P. Acharya, Parsa M. Nafisi, Austin Gardner, Joanna L. MacKay, Kousik Kundu, Sanjay Kumar, Niren Murthy

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Original languageEnglish (US)
Pages (from-to)10379-10381
Number of pages3
JournalChemical Communications
Volume49
Issue number88
DOIs
StatePublished - Oct 10 2013
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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