A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Abhinav P. Acharya, Parsa M. Nafisi, Austin Gardner, Joanna L. MacKay, Kousik Kundu, Sanjay Kumar, Niren Murthy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Original languageEnglish (US)
Pages (from-to)10379-10381
Number of pages3
JournalChemical Communications
Volume49
Issue number88
DOIs
StatePublished - Nov 14 2013
Externally publishedYes

Fingerprint

Immunosorbents
Peroxidase
Assays
Enzymes
Horseradish Peroxidase
Medicine
Reactive Oxygen Species
Oxygen

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude. / Acharya, Abhinav P.; Nafisi, Parsa M.; Gardner, Austin; MacKay, Joanna L.; Kundu, Kousik; Kumar, Sanjay; Murthy, Niren.

In: Chemical Communications, Vol. 49, No. 88, 14.11.2013, p. 10379-10381.

Research output: Contribution to journalArticle

Acharya, AP, Nafisi, PM, Gardner, A, MacKay, JL, Kundu, K, Kumar, S & Murthy, N 2013, 'A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude', Chemical Communications, vol. 49, no. 88, pp. 10379-10381. https://doi.org/10.1039/c3cc44783a
Acharya, Abhinav P. ; Nafisi, Parsa M. ; Gardner, Austin ; MacKay, Joanna L. ; Kundu, Kousik ; Kumar, Sanjay ; Murthy, Niren. / A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude. In: Chemical Communications. 2013 ; Vol. 49, No. 88. pp. 10379-10381.
@article{422d9aedde554b2d91a12fd727cfc2d7,
title = "A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude",
abstract = "The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.",
author = "Acharya, {Abhinav P.} and Nafisi, {Parsa M.} and Austin Gardner and MacKay, {Joanna L.} and Kousik Kundu and Sanjay Kumar and Niren Murthy",
year = "2013",
month = "11",
day = "14",
doi = "10.1039/c3cc44783a",
language = "English (US)",
volume = "49",
pages = "10379--10381",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "88",

}

TY - JOUR

T1 - A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

AU - Acharya, Abhinav P.

AU - Nafisi, Parsa M.

AU - Gardner, Austin

AU - MacKay, Joanna L.

AU - Kundu, Kousik

AU - Kumar, Sanjay

AU - Murthy, Niren

PY - 2013/11/14

Y1 - 2013/11/14

N2 - The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

AB - The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

UR - http://www.scopus.com/inward/record.url?scp=84885466889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885466889&partnerID=8YFLogxK

U2 - 10.1039/c3cc44783a

DO - 10.1039/c3cc44783a

M3 - Article

C2 - 24071916

AN - SCOPUS:84885466889

VL - 49

SP - 10379

EP - 10381

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 88

ER -