A fed-batch technique to evaluate biodegradation rates of inhibitory compounds with anaerobic biofilms attached to granular activated carbon

Peter Fox, M. T. Suidan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Adsorption kinetics render measurements of substrate utilization rates in biofilms attached to adsorbing materials difficult. A fed-batch technique which maintains a constant concentration of substrate in the presence of biomass attached to granular activated carbon (GAC) was developed to measure biological kinetics independent of adsorption kinetics. The fed-batch technique used pre-adsorbed substrate on GAC as a source of substrate. During the experiment, the mass of substrate that is biodegraded is negligible when compared to the large mass of adsorbed substrate. Near equilibrium adsorption phenomena maintains the concentration of substrate constant throughout the experiment and eliminates mass transport resistance within the biofilms. Substrate utilization rates were measured at a specific substrate concentration by monitoring methane gas production rates. Samples of GAC with attached biomass were removed from two expanded-bed anaerobic reactors. GAC samples from a 3-ethylphenol (3-ep) fed reactor were used in batch experiments with 3-ep as the substrate and GAC samples from an orthochlorophenol (OCP) fed reactor were used in batch experiments with OCP as the substrate. Data were observed to fit Haldane kinetics and predictions from the Haldane kinetic parameters were in agreement with transient behavior from the 3-ep fed reactor. This technique was useful in predicting threshold inhibitory levels for continuous treatment of inhibitory wastewaters in expanded-bed GAC reactors. The fed-batch technique might also be applied to measure substrate utilization rates of biomass attached to other adsorbable materials such as soils.

Original languageEnglish (US)
Title of host publicationWater Science and Technology
Pages1337-1346
Number of pages10
Volume23
Edition7-9
StatePublished - 1991
Externally publishedYes
EventProceedings of the 15th Biennial Conference of the International Association on Water Pollution Research and Control - Kyoto, Jpn
Duration: Jul 29 1990Aug 3 1990

Other

OtherProceedings of the 15th Biennial Conference of the International Association on Water Pollution Research and Control
CityKyoto, Jpn
Period7/29/908/3/90

Fingerprint

activated carbon
biofilm
biodegradation
substrate
kinetics
adsorption
rate
biomass
experiment
mass transport
gas production
methane
reactor
wastewater

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

A fed-batch technique to evaluate biodegradation rates of inhibitory compounds with anaerobic biofilms attached to granular activated carbon. / Fox, Peter; Suidan, M. T.

Water Science and Technology. Vol. 23 7-9. ed. 1991. p. 1337-1346.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fox, P & Suidan, MT 1991, A fed-batch technique to evaluate biodegradation rates of inhibitory compounds with anaerobic biofilms attached to granular activated carbon. in Water Science and Technology. 7-9 edn, vol. 23, pp. 1337-1346, Proceedings of the 15th Biennial Conference of the International Association on Water Pollution Research and Control, Kyoto, Jpn, 7/29/90.
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