A dominant selectable marker that is meiotically stable in Neurospora crassa: the amdS gene of Aspergillus nidulans

Carl T. Yamashiro, Oded Yarden, Charles Yanofsky

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

When Neurospora crassa is transformed using a Neurospora gene as the selectable marker, the vegetatively stable transformants obtained cannot be used successfully in a cross because the selectable marker will be inactivated by the process of RIP (repeat-induced point mutation). Introduction of the acetamidase-encoding gene amdS of Aspergillus nidulans into N. crassa by transformation yielded transformants that would grow in minimal medium containing acetamide as a sole nitrogen source. In mitotically stable transformants containing a single copy of the amdS gene, the capacity to utilize acetamide as a sole nitrogen source was maintained in the progeny of a sexual cross. Therefore, the A. nidulans amdS gene is an appropriate dominant selectable marker for use in transformation analyses with N. crassa in which sexual crosses will be subsequently performed.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalMGG Molecular & General Genetics
Volume236
Issue number1
DOIs
StatePublished - Dec 1 1992

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Keywords

  • Acetamidase
  • DNA-mediated transformation
  • Neurospora crassa
  • Repeat-induced point mutation (RIP)
  • amdS gene

ASJC Scopus subject areas

  • Genetics

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