A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning

David M. Ash, Jennifer Hackney Price, Michele Jean-Francois, Neal C. Burton, Leonard L. Dobens

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The bunched (bun) gene encodes the Drosophila member of the TSC-22/GILZ family of leucine zipper transcriptional regulators. The bun locus encodes multiple BUN protein isoforms and has diverse roles during patterning of the eye, wing margin, dorsal notum and eggshell. Here we report the construction and activity of a dominant negative allele (BunDN) of the BUN-B isoform. In the ovary, BunDN expression in the follicle cells (FC) resulted in epithelial defects including aberrant accumulation of DE-cadherin and failure to rearrange into columnar FC cell shapes. BunDN expression in the posterior FC led to loss of epithelial integrity associated with extensive apoptosis. BunDN FC phenotypes collectively resemble loss-of-function bun mutant phenotypes. BunDN expression using tissue-specific imaginal disk drivers resulted in characteristic cuticular patterning defects that were enhanced by bun mutations and suppressed by co-expression of the BUN-B protein isoform. These data indicate that BunDN has dominant negative activity useful to identify bun functions and genetic interactions that occur during tissue patterning.

Original languageEnglish (US)
Pages (from-to)559-569
Number of pages11
JournalMechanisms of Development
Volume124
Issue number7-8
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

Leucine Zippers
Blood Urea Nitrogen
Alleles
Protein Isoforms
Egg Shell
Imaginal Discs
Phenotype
Cell Shape
Cadherins
Drosophila
Ovary
Apoptosis
Mutation
Drosophila zipper protein
Genes

Keywords

  • Bunched
  • Dominant negative
  • Drosophila
  • GILZ
  • Oogenesis
  • Tissue patterning
  • TSC-22
  • TSC-22/GILZ family

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning. / Ash, David M.; Hackney Price, Jennifer; Jean-Francois, Michele; Burton, Neal C.; Dobens, Leonard L.

In: Mechanisms of Development, Vol. 124, No. 7-8, 08.2007, p. 559-569.

Research output: Contribution to journalArticle

Ash, David M. ; Hackney Price, Jennifer ; Jean-Francois, Michele ; Burton, Neal C. ; Dobens, Leonard L. / A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning. In: Mechanisms of Development. 2007 ; Vol. 124, No. 7-8. pp. 559-569.
@article{c409c488a59144bda873b3d7088c8d4f,
title = "A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning",
abstract = "The bunched (bun) gene encodes the Drosophila member of the TSC-22/GILZ family of leucine zipper transcriptional regulators. The bun locus encodes multiple BUN protein isoforms and has diverse roles during patterning of the eye, wing margin, dorsal notum and eggshell. Here we report the construction and activity of a dominant negative allele (BunDN) of the BUN-B isoform. In the ovary, BunDN expression in the follicle cells (FC) resulted in epithelial defects including aberrant accumulation of DE-cadherin and failure to rearrange into columnar FC cell shapes. BunDN expression in the posterior FC led to loss of epithelial integrity associated with extensive apoptosis. BunDN FC phenotypes collectively resemble loss-of-function bun mutant phenotypes. BunDN expression using tissue-specific imaginal disk drivers resulted in characteristic cuticular patterning defects that were enhanced by bun mutations and suppressed by co-expression of the BUN-B protein isoform. These data indicate that BunDN has dominant negative activity useful to identify bun functions and genetic interactions that occur during tissue patterning.",
keywords = "Bunched, Dominant negative, Drosophila, GILZ, Oogenesis, Tissue patterning, TSC-22, TSC-22/GILZ family",
author = "Ash, {David M.} and {Hackney Price}, Jennifer and Michele Jean-Francois and Burton, {Neal C.} and Dobens, {Leonard L.}",
year = "2007",
month = "8",
doi = "10.1016/j.mod.2007.05.003",
language = "English (US)",
volume = "124",
pages = "559--569",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "7-8",

}

TY - JOUR

T1 - A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning

AU - Ash, David M.

AU - Hackney Price, Jennifer

AU - Jean-Francois, Michele

AU - Burton, Neal C.

AU - Dobens, Leonard L.

PY - 2007/8

Y1 - 2007/8

N2 - The bunched (bun) gene encodes the Drosophila member of the TSC-22/GILZ family of leucine zipper transcriptional regulators. The bun locus encodes multiple BUN protein isoforms and has diverse roles during patterning of the eye, wing margin, dorsal notum and eggshell. Here we report the construction and activity of a dominant negative allele (BunDN) of the BUN-B isoform. In the ovary, BunDN expression in the follicle cells (FC) resulted in epithelial defects including aberrant accumulation of DE-cadherin and failure to rearrange into columnar FC cell shapes. BunDN expression in the posterior FC led to loss of epithelial integrity associated with extensive apoptosis. BunDN FC phenotypes collectively resemble loss-of-function bun mutant phenotypes. BunDN expression using tissue-specific imaginal disk drivers resulted in characteristic cuticular patterning defects that were enhanced by bun mutations and suppressed by co-expression of the BUN-B protein isoform. These data indicate that BunDN has dominant negative activity useful to identify bun functions and genetic interactions that occur during tissue patterning.

AB - The bunched (bun) gene encodes the Drosophila member of the TSC-22/GILZ family of leucine zipper transcriptional regulators. The bun locus encodes multiple BUN protein isoforms and has diverse roles during patterning of the eye, wing margin, dorsal notum and eggshell. Here we report the construction and activity of a dominant negative allele (BunDN) of the BUN-B isoform. In the ovary, BunDN expression in the follicle cells (FC) resulted in epithelial defects including aberrant accumulation of DE-cadherin and failure to rearrange into columnar FC cell shapes. BunDN expression in the posterior FC led to loss of epithelial integrity associated with extensive apoptosis. BunDN FC phenotypes collectively resemble loss-of-function bun mutant phenotypes. BunDN expression using tissue-specific imaginal disk drivers resulted in characteristic cuticular patterning defects that were enhanced by bun mutations and suppressed by co-expression of the BUN-B protein isoform. These data indicate that BunDN has dominant negative activity useful to identify bun functions and genetic interactions that occur during tissue patterning.

KW - Bunched

KW - Dominant negative

KW - Drosophila

KW - GILZ

KW - Oogenesis

KW - Tissue patterning

KW - TSC-22

KW - TSC-22/GILZ family

UR - http://www.scopus.com/inward/record.url?scp=34547215347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547215347&partnerID=8YFLogxK

U2 - 10.1016/j.mod.2007.05.003

DO - 10.1016/j.mod.2007.05.003

M3 - Article

VL - 124

SP - 559

EP - 569

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 7-8

ER -