A DNA-damaging oxoaporphine alkaloid from Piper caninum

Ji Ma, Shannon H. Jones, Rebekah Marshall, Randall K. Johnson, Sidney Hecht

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bioassay-guided fractionation of an active organic extract of Piper caninum, using a sensitive yeast assay to monitor putative double-strand DNA-damaging activity, resulted in the isolation of the 4,5-dioxoaporphine alkaloid cepharadione A (1). Compound 1 exhibited potent inhibitory activity in a yeast cytotoxicity assay with IC50 values of 50.2 nM toward RS321NpRAD52 grown on glucose versus 293 nM toward the same yeast strain grown on galactose.

Original languageEnglish (US)
Pages (from-to)1162-1164
Number of pages3
JournalJournal of Natural Products
Volume67
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

A-DNA
Piper
Alkaloids
Yeast
alkaloids
Yeasts
yeasts
Assays
DNA
Bioassay
assays
Cytotoxicity
Fractionation
Galactose
Biological Assay
galactose
Inhibitory Concentration 50
inhibitory concentration 50
cytotoxicity
fractionation

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

A DNA-damaging oxoaporphine alkaloid from Piper caninum. / Ma, Ji; Jones, Shannon H.; Marshall, Rebekah; Johnson, Randall K.; Hecht, Sidney.

In: Journal of Natural Products, Vol. 67, No. 7, 07.2004, p. 1162-1164.

Research output: Contribution to journalArticle

Ma, J, Jones, SH, Marshall, R, Johnson, RK & Hecht, S 2004, 'A DNA-damaging oxoaporphine alkaloid from Piper caninum', Journal of Natural Products, vol. 67, no. 7, pp. 1162-1164. https://doi.org/10.1021/np040056x
Ma, Ji ; Jones, Shannon H. ; Marshall, Rebekah ; Johnson, Randall K. ; Hecht, Sidney. / A DNA-damaging oxoaporphine alkaloid from Piper caninum. In: Journal of Natural Products. 2004 ; Vol. 67, No. 7. pp. 1162-1164.
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