A Disposable Tear Glucose Biosensor—Part 5: Improvements in Reagents and Tear Sampling Component

Chi En Lin, Yuka Ito, Anna Deng, Jared Johns, Daniel Matloff, Curtiss B. Cook, Koji Sode, Jeffrey LaBelle

Research output: Contribution to journalArticle

Abstract

A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower Km may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.

Original languageEnglish (US)
JournalJournal of diabetes science and technology
DOIs
StateAccepted/In press - Apr 1 2018

Fingerprint

Glucose sensors
Glucose 1-Dehydrogenase
Tears
Glucose
Sampling
Contact lenses
Monitoring
Sensors
Acetaminophen
Uric Acid
Ascorbic Acid
Ascorbic acid
Enzymes
Contact Lenses
Acids

Keywords

  • diabetes mellitus
  • glucose dehydrogenase
  • integrated tear glucose sensor
  • noninvasive glucose monitoring
  • Schirmer’s test strip

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Bioengineering
  • Biomedical Engineering

Cite this

A Disposable Tear Glucose Biosensor—Part 5 : Improvements in Reagents and Tear Sampling Component. / Lin, Chi En; Ito, Yuka; Deng, Anna; Johns, Jared; Matloff, Daniel; Cook, Curtiss B.; Sode, Koji; LaBelle, Jeffrey.

In: Journal of diabetes science and technology, 01.04.2018.

Research output: Contribution to journalArticle

Lin, Chi En ; Ito, Yuka ; Deng, Anna ; Johns, Jared ; Matloff, Daniel ; Cook, Curtiss B. ; Sode, Koji ; LaBelle, Jeffrey. / A Disposable Tear Glucose Biosensor—Part 5 : Improvements in Reagents and Tear Sampling Component. In: Journal of diabetes science and technology. 2018.
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