TY - JOUR
T1 - A Disposable Tear Glucose Biosensor—Part 5
T2 - Improvements in Reagents and Tear Sampling Component
AU - Lin, Chi En
AU - Ito, Yuka
AU - Deng, Anna
AU - Johns, Jared
AU - Matloff, Daniel
AU - Cook, Curtiss B.
AU - Sode, Koji
AU - LaBelle, Jeffrey
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the Mayo Clinic.
Funding Information:
The authors would like to thank Simran Shrama and Shreya Kodira from PES University, Bangalore for assisting with data collection. Yuka Ito was supported by the Japan Student Services Organization (JASSO).
Funding Information:
The authors would like to thank Simran Shrama and Shreya Kodira from PES University, Bangalore for assisting with data collection. Yuka Ito was supported by the Japan Student Services Organization (JASSO). The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the Mayo Clinic.
Publisher Copyright:
© 2018, 2018 Diabetes Technology Society.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower K m may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.
AB - A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose. Expanding from previous work, an improved TG sensor that implements dried reagents, genetically modified glucose dehydrogenase (GDH), and a tear sampler was developed and compared against the TG sensor prepared with commercial GDH. It was found that neither sensor was affected by the tear interferents: ascorbic acid, acetaminophen, and uric acid. The sensor prepared with commercial GDH generated higher current. This suggests that using enzymes with lower K m may be advantageous when operating in low glucose environments like tears. The improved TG sensor also demonstrated the potential of integrating Schirmer’s test strip as a tear sampler for self-monitoring of TG.
KW - Schirmer’s test strip
KW - diabetes mellitus
KW - glucose dehydrogenase
KW - integrated tear glucose sensor
KW - noninvasive glucose monitoring
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U2 - 10.1177/1932296818769944
DO - 10.1177/1932296818769944
M3 - Article
C2 - 29667855
AN - SCOPUS:85045829931
SN - 1932-2968
VL - 12
SP - 842
EP - 846
JO - Journal of diabetes science and technology
JF - Journal of diabetes science and technology
IS - 4
ER -