A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy

Jeffrey LaBelle, Kinjal Bhavsar, Aaron Fairchild, Anusuya Das, James Sweeney, Terry Alford, Joseph Wang, Veer P. Bhavanandan, Lokesh Joshi

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A biosensor for the serum cytokine, interleukin-12 (IL-12), based upon a label-free electrochemical impedance spectroscopy monitoring is described. Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, <100 fM with p < 0.05 in a label-free and real-time manner. The cost-effective approach described here can be used for diagnosis of diseases (like MS) with known biomarkers in body fluids and for monitoring physiological levels of biomolecules with healthcare, food, and environmental relevance.

Original languageEnglish (US)
Pages (from-to)428-431
Number of pages4
JournalBiosensors and Bioelectronics
Volume23
Issue number3
DOIs
StatePublished - Oct 31 2007

Keywords

  • Disease diagnosis
  • Impedance
  • Interleukins
  • Label-free

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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