A core linkage map of the bumblebee Bombus terrestris

Lena Wilfert, Juergen Gadau, Paul Schmid-Hempel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The bumblebee Bombus terrestris is an economically important pollinator and an emerging model species in quantitative and population genetics. We generated genetic linkage maps for 3 independent mapping populations of B. terrestris. The linkage map with the highest resolution had 21 linkage groups, which adequately represents the haploid chromosome number of B. terrestris (n = 18). This map can be considered saturated, with an average marker distance of 10.3 cM and an estimated genome coverage of 81%. Using flow cytometry, we have estimated the genome size of this species to be 625 Mb. With an estimated total recombination genome length of 2760 cM, this results in a ratio of 226 kb/cM between the physical and genetic genome sizes. A recurring set of microsatellites and amplified fragment length polymorphism (AFLP) markers allowed the alignment of 14 linkage groups between the 3 maps. We propose to adopt this core map as a reference tool for future genetic and molecular work in B. terrestris.

Original languageEnglish (US)
Pages (from-to)1215-1226
Number of pages12
JournalGenome
Volume49
Issue number10
DOIs
StatePublished - Oct 2006

Fingerprint

Genome Size
Bombus terrestris
Bombus
chromosome mapping
Genome
Chromosomes, Human, 4-5
Genetic Linkage
genome
Haploidy
Population Genetics
linkage groups
Microsatellite Repeats
Genetic Recombination
Molecular Biology
Flow Cytometry
quantitative genetics
pollinating insects
haploidy
Population
chromosome number

Keywords

  • AFLP
  • Genome mapping
  • Microsatellite
  • RAPD
  • Recombination frequency

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

A core linkage map of the bumblebee Bombus terrestris. / Wilfert, Lena; Gadau, Juergen; Schmid-Hempel, Paul.

In: Genome, Vol. 49, No. 10, 10.2006, p. 1215-1226.

Research output: Contribution to journalArticle

Wilfert, L, Gadau, J & Schmid-Hempel, P 2006, 'A core linkage map of the bumblebee Bombus terrestris', Genome, vol. 49, no. 10, pp. 1215-1226. https://doi.org/10.1139/G06-075
Wilfert, Lena ; Gadau, Juergen ; Schmid-Hempel, Paul. / A core linkage map of the bumblebee Bombus terrestris. In: Genome. 2006 ; Vol. 49, No. 10. pp. 1215-1226.
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