A core linkage map of the bumblebee Bombus terrestris

Lena Wilfert, Juergen Gadau, Paul Schmid-Hempel

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The bumblebee Bombus terrestris is an economically important pollinator and an emerging model species in quantitative and population genetics. We generated genetic linkage maps for 3 independent mapping populations of B. terrestris. The linkage map with the highest resolution had 21 linkage groups, which adequately represents the haploid chromosome number of B. terrestris (n = 18). This map can be considered saturated, with an average marker distance of 10.3 cM and an estimated genome coverage of 81%. Using flow cytometry, we have estimated the genome size of this species to be 625 Mb. With an estimated total recombination genome length of 2760 cM, this results in a ratio of 226 kb/cM between the physical and genetic genome sizes. A recurring set of microsatellites and amplified fragment length polymorphism (AFLP) markers allowed the alignment of 14 linkage groups between the 3 maps. We propose to adopt this core map as a reference tool for future genetic and molecular work in B. terrestris.

Original languageEnglish (US)
Pages (from-to)1215-1226
Number of pages12
JournalGenome
Volume49
Issue number10
DOIs
StatePublished - Oct 2006

Keywords

  • AFLP
  • Genome mapping
  • Microsatellite
  • RAPD
  • Recombination frequency

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'A core linkage map of the bumblebee Bombus terrestris'. Together they form a unique fingerprint.

Cite this