A computerized method of determining the number of synaptic contacts in a volume of cerebral cortex

Mark J. West, Paul D. Coleman, Urs R. Wyss

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A computerized flying‐spot scanning device has been used to count the number of synaptic contacts in neural tissue stained with ethanolic phosphotungstic acid (E‐PTA). The ability of the computer to idealize the irregular synaptic contacts as solid disks by modifying the information gleaned from scanning the preparation allows the stereological principle of Weibel & Gomez to be used to calculate the number of E‐PTA stained synaptic contacts per unit volume of tissue. This technique represents a significant improvement over the time necessary for making similar quantitative determinations manually. 1972 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalJournal of Microscopy
Volume95
Issue number2
DOIs
StatePublished - Apr 1972
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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