A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia

L. W. Beukeboom, O. Niehuis, B. A. Pannebakker, T. Koevoets, J. D. Gibson, D. M. Shuker, L. Van De Zande, Juergen Gadau

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We present the first intraspecific linkage map for Nasonia vitripennis based on molecular markers. The map consists of 36 new microsatellite markers, extracted from the Nasonia genome sequence, and spans 515 cM. The five inferred linkage groups correspond to the five chromosomes of Nasonia. Comparison of recombination frequencies of the marker intervals spread over the whole genome (N = 33 marker intervals) between the intraspecific N. vitripennis map and an interspecific N. vitripennis × N. giraulti map revealed a slightly higher (1.8%) recombination frequency in the intraspecific cross. We further considered an N. vitripennis × N. longicornis map with 29 microsatellite markers spanning 430 cM. Recombination frequencies in the two interspecific crosses differed neither between reciprocal crosses nor between mapping populations of embryos and adults. No major chromosomal rearrangements were found for the analyzed genomic segments. The observed differential F 2 hybrid male mortality has no significant effect on the genome-wide recombination frequency in Nasonia. We conclude that interspecific crosses between the different Nasonia species, a hallmark of Nasonia genetics, are generally suitable for mapping quantitative and qualitative trait loci for species differences.

Original languageEnglish (US)
Pages (from-to)302-309
Number of pages8
JournalHeredity
Volume104
Issue number3
DOIs
StatePublished - Mar 2010

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Genetic Recombination
Genome
Microsatellite Repeats
Population
Quantitative Trait Loci
Embryonic Structures
Chromosomes
Mortality

Keywords

  • Hybrids
  • Insects
  • Linkage map
  • Microsatellites
  • Recombination
  • Speciation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Beukeboom, L. W., Niehuis, O., Pannebakker, B. A., Koevoets, T., Gibson, J. D., Shuker, D. M., ... Gadau, J. (2010). A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia. Heredity, 104(3), 302-309. https://doi.org/10.1038/hdy.2009.185

A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia. / Beukeboom, L. W.; Niehuis, O.; Pannebakker, B. A.; Koevoets, T.; Gibson, J. D.; Shuker, D. M.; Van De Zande, L.; Gadau, Juergen.

In: Heredity, Vol. 104, No. 3, 03.2010, p. 302-309.

Research output: Contribution to journalArticle

Beukeboom, LW, Niehuis, O, Pannebakker, BA, Koevoets, T, Gibson, JD, Shuker, DM, Van De Zande, L & Gadau, J 2010, 'A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia', Heredity, vol. 104, no. 3, pp. 302-309. https://doi.org/10.1038/hdy.2009.185
Beukeboom LW, Niehuis O, Pannebakker BA, Koevoets T, Gibson JD, Shuker DM et al. A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia. Heredity. 2010 Mar;104(3):302-309. https://doi.org/10.1038/hdy.2009.185
Beukeboom, L. W. ; Niehuis, O. ; Pannebakker, B. A. ; Koevoets, T. ; Gibson, J. D. ; Shuker, D. M. ; Van De Zande, L. ; Gadau, Juergen. / A comparison of recombination frequencies in intraspecific versus interspecific mapping populations of Nasonia. In: Heredity. 2010 ; Vol. 104, No. 3. pp. 302-309.
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